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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Effects of light on development of mammalian zygotes
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Effects of light on development of mammalian zygotes

机译：光对哺乳动物合子发育的影响

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摘要

It is generally assumed that light has no effect on the physiology of oocytes, zygotes, or early embryos. Therefore, little or no attention has been paid to lighting conditions during the handling of these cells in vitro. Here we show that cool white fluorescent light, rich in short-wavelength visible light and commonly used in research and clinical laboratories, produces more reactive oxygen species in mouse and hamster zygotes than does warm white fluorescent light. Mouse blastocysts that developed from zygotes shielded from light best developed to term fetuses followed by those exposed to warm white fluorescent light and then by those exposed to cool white fluorescent light. We hypothesized that light is one of the physical factors affecting embryonic environment and that its effects on cultured mammalian zygotes and embryos should not be overlooked.
机译：通常认为光对卵母细胞，合子或早期胚胎的生理没有影响。因此，在体外处理这些细胞的过程中很少或根本没有注意光照条件。在这里，我们显示冷白光荧光灯，富含短波可见光，通常在研究和临床实验室中使用，比暖白光荧光灯在小鼠和仓鼠受精卵中产生更多的活性氧。由受光屏蔽的合子发育的小鼠胚泡最好发育为足月胎儿，其次是暴露于温暖的白色荧光灯的小鼠，然后是暴露于冷白色荧光灯的胎儿。我们假设光是影响胚胎环境的物理因素之一，并且它对培养的哺乳动物合子和胚胎的影响不容忽视。

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