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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >PfEMP1-DBL1α amino acid motifs in severe disease states of Plasmodium falciparum malaria
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PfEMP1-DBL1α amino acid motifs in severe disease states of Plasmodium falciparum malaria

机译：恶性疟原虫疟疾的严重疾病状态下的PfEMP1-DBL1α氨基酸基序

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摘要

An infection with Plasmodium falciparum may lead to severe malaria as a result of excessive binding of infected erythrocytes in the microvasculature. Vascular adhesion is mediated by P. falciparum erythrocyte membrane protein-1 (PfEMP1), which is encoded for by highly polymorphic members of the var-gene family. Here, we profile var gene transcription in fresh P. falciparum trophozo-ites from Ugandan children with malaria through var-specific DBL1α-PCR amplification and sequencing. A method for subsec-tioning region alignments into homology areas (MOTIFF) was developed to examine collected sequences. Specific PfEMP1-DBL1α amino acid motifs correlated with rosetting and severe malaria, with motif location corresponding to distinct regions of receptor interaction. The method is potentially applicable to other families of variant proteins and may be useful in identifying sequence-phenotype relationships. The results suggest that certain PfEMP1 sequences are predisposed to inducing severe malaria.
机译：由于微血管系统中受感染红细胞的过度结合，恶性疟原虫感染可能导致严重的疟疾。血管粘附是由恶性疟原虫红细胞膜蛋白1（PfEMP1）介导的，它由var基因家族的高度多态性成员编码。在这里，我们通过var-specificDBL1α-PCR扩增和测序分析来自乌干达疟疾儿童的新鲜恶性疟原虫滋养体中的var基因转录。开发了一种将区域比对细分为同源区域的方法（MOTIFF），以检查收集到的序列。特定的PfEMP1-DBL1α氨基酸基序与玫瑰结和严重疟疾相关，基序位置对应于受体相互作用的不同区域。该方法可能适用于变异蛋白的其他家族，并且可用于鉴定序列-表型关系。结果表明某些PfEMP1序列易诱发严重疟疾。

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