...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Hyperpolarized ~(13)C allows a direct measure of flux through a single enzyme-catalyzed step by NMR
【24h】

Hyperpolarized ~(13)C allows a direct measure of flux through a single enzyme-catalyzed step by NMR

机译：超极化〜（13）C可通过NMR直接测量通过单个酶催化步骤的通量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

~(13)C NMR is a powerful tool for monitoring metabolic fluxes in vivo. The recent availability of automated dynamic nuclear polarization equipment for hyperpolarizing ~(13)C nuclei now offers the potential to measure metabolic fluxes through select enzyme-catalyzed steps with substantially improved sensitivity. Here, we investigated the metabolism of hyperpolarized [1-~(13)C_1]pyruvate in a widely used model for physiology and pharmacology, the perfused rat heart. Dissolved ~(13)CO_2, the immediate product of the first step of the reaction catalyzed by pyruvate dehydrogenase, was observed with a temporal resolution of ≈1 s along with H~(13)CO_3~-, the hydrated form of ~(13)CO_2 generated catalytically by carbonic anhy-drase. In hearts presented with the medium-chain fatty acid oc-tanoate in addition to hyperpolarized [1-~(13)C_1]pyruvate, production of ~(13)CO_2 and H~(13)CO_3~- was suppressed by ≈90%, whereas the signal from [1-~(13)C_1]lactate was enhanced. In separate experiments, it was shown that O_2 consumption and tricarboxylic acid (TCA) cycle flux were unchanged in the presence of added octanoate. Thus, the rate of appearance of ~(13)CO_2 and H~(13)CO_3~- from [1-~(13)C_1]pyruvate does not reflect production of CO_2 in the TCA cycle but rather reflects flux through pyruvate dehydrogenase exclusively.
机译：〜（13）C NMR是监测体内代谢通量的有力工具。用于超极化〜（13）C核的自动动态核极化设备的最新可用性现在提供了潜力，可通过精选的酶催化步骤以显着提高的灵敏度测量代谢通量。在这里，我们在生理和药理学广泛使用的模型（灌流的大鼠心脏）中研究了超极化[1-〜（13）C_1]丙酮酸的代谢。丙酮酸脱氢酶催化的第一步反应的直接产物〜（13）CO_2与H〜（13）CO_3〜-（〜（13）的水合形式）的时间分辨度约为≈1s。碳酸酐吸收催化生成的）CO_2在心脏中，除了超极化的[1-〜（13）C_1]丙酮酸盐外，还存在中链脂肪酸辛酸脂肪酸，〜（13）CO_2和H〜（13）CO_3〜-的产生被抑制了≈90％ ，而[1-〜（13）C_1]乳酸盐的信号增强。在单独的实验中，表明在添加辛酸酯的情况下，O_2的消耗量和三羧酸（TCA）循环通量没有变化。因此，[1-〜（13）C_1]丙酮酸盐中〜（13）CO_2和H〜（13）CO_3〜-的出现速率并不反映TCA循环中CO_2的产生，而仅反映了通过丙酮酸脱氢酶的通量。 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号