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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Prion strain discrimination in cell culture: The cell panel assay
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Prion strain discrimination in cell culture: The cell panel assay

机译：细胞培养中的on病毒菌株鉴别：细胞组测定

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摘要

Prions are thought to consist mainly or entirely of misfolded PrP, a constitutively expressed host protein. Prions associated with the same PrP sequence may occur in the form of different strains; the strain phenotype is believed to be encoded by the conformation of the PrP. Some cell lines can be persistently infected by prions and, interestingly, show preference for certain strains. We report that a cloned murine neuroblastoma cell population, N2a-PK1, is highly heterogeneous in regard to its susceptibility to RML and 22L prions. Remarkably, sibling subclones may show very different relative susceptibilities to the two strains, indicating that the responses can vary independently. We have assembled four cell lines, N2a-PK1, N2a-R33, LD9 and CAD5, which show widely different responses to prion strains RML, 22L, 301C, and Me7, into a panel that allows their discrimination in vitro within 2 weeks, using the standard scrapie cell assay (SSCA).
机译：ions病毒被认为主要或完全由错误折叠的PrP（组成性表达的宿主蛋白）组成。与相同PrP序列相关的病毒可能以不同毒株的形式出现。据信菌株表型由PrP的构象编码。某些细胞系可以被病毒持续感染，有趣的是，它们对某些菌株表现出偏爱。我们报告说，一个克隆的鼠神经母细胞瘤细胞群体，N2a-PK1，对RML和22L pr病毒的敏感性很高。值得注意的是，同胞亚克隆可能对两种菌株表现出截然不同的相对敏感性，表明反应可以独立变化。我们将四个细胞系N2a​​-PK1，N2a-R33，LD9和CAD5组装在一起，将对病毒菌株RML，22L，301C和Me7表现出截然不同的反应，使用标准的瘙痒病细胞检测（SSCA）。

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