...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Manipulating phospholipids for crystallization of a membrane transport protein
【24h】

Manipulating phospholipids for crystallization of a membrane transport protein

机译：操纵磷脂使膜转运蛋白结晶

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Crystallization is a major bottleneck to obtaining x-ray structures of membrane proteins. By applying an established crystallization protocol for the lactose permease (LacY) of Escherichia coli, a systematic study of the effect of phospholipids (PL) on crystallization of LacY was undertaken. We observe three different crystal forms that diffract to increasingly better resolution in a manner that correlates with the concentration of copurified PL. Consistently, progressive addition of E. coli PL to delipidated LacY leads to different crystal forms. Tetragonal crystals are obtained with improved diffraction quality for a stable mutant by carefully adjusting PL content. Furthermore, crystals of good quality from wild-type LacY, a particularly difficult protein, were also obtained by using same approach. Thus, it is likely that manipulation of PL is a good strategy for predominantly hydrophobic membrane proteins like LacY.
机译：结晶是获得膜蛋白X射线结构的主要瓶颈。通过为大肠杆菌的乳糖通透酶（LacY）应用已建立的结晶方案，对磷脂（PL）对LacY结晶的影响进行了系统的研究。我们观察到三种不同的晶体形式，它们以与共纯化的PL浓度相关的方式衍射到越来越高的分辨率。一致地，将大肠杆菌PL逐步添加到脱脂的LacY中会导致形成不同的晶体形式。通过仔细调节PL含量，可得到具有改进的衍射质量的四方晶体，以获得稳定的突变体。此外，还通过使用相同的方法从野生型LacY（一种特别困难的蛋白质）中获得了高质量的晶体。因此，对于主要是疏水性膜蛋白（如LacY）的PL操纵可能是一个很好的策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号