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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor
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Microtubule-associated protein 2 (MAP2) is a neurosteroid receptor

机译：微管相关蛋白2（MAP2）是神经甾体受体

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摘要

The neurosteroid pregnenolone (PREG) and its chemically synthesized analog 3β-methoxypregnenolone (MePREG) bind to micro-tubule-associated protein 2 (MAP2) and stimulate the polymerization of microtubules. PREG, MePREG, and progesterone (PROG; the physiological immediate metabolite of PREG) significantly enhance neurite outgrowth of nerve growth factor-pretreated PC12 cells. However, PROG, although it binds to MAP2, does not increase the immunostaining of MAP2, contrary to PREG and MePREG. Nocoda-zole, a microtubule-disrupting agent, induces a major retraction of neurites in control cultures, but pretreatment with PREG MePREG is protective. Decreasing MAP2 expression by RNA interference does not modify PROG action, but it prevents the stimulatory effects of PREG and MePREG on neurite extension, showing that MAP2 is their specific receptor.
机译：神经甾体孕烯醇酮（PREG）及其化学合成的类似物3β-甲氧基孕烯醇酮（MePREG）与微管相关蛋白2（MAP2）结合并刺激微管的聚合。 PREG，MePREG和孕酮（PROG； PREG的生理直接代谢物）显着增强了神经生长因子预处理的PC12细胞的神经突向外生长。但是，尽管PROG与MAP2结合，但与PREG和MePREG相反，它并未增加MAP2的免疫染色。 Nocoda-zole是一种微管破坏剂，可诱导对照培养物中的神经突大量回缩，但使用PREG MePREG进行预处理具有保护性。 RNA干扰降低MAP2的表达不会改变PROG的作用，但可以阻止PREG和MePREG对神经突延伸的刺激作用，表明MAP2是其特异性受体。

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