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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Surprising contribution to aminoacylation and translation of non-Watson-Crick pairs in tRNA
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Surprising contribution to aminoacylation and translation of non-Watson-Crick pairs in tRNA

机译：对tRNA中非Watson-Crick对的氨基酰化和翻译的惊人贡献

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摘要

Molecules of transfer RNA (tRNA) typically contain four stems composed of Watson-Crick (W-C) base pairs and infrequent mis-pairs such as G-U and A-C. The latter mispairs are fundamental units of RNA secondary structure found in nearly every class of RNA and are nearly isomorphic to W-C pairs. Therefore, they often substitute for G-C or A-U base pairs. The mispairs also have unique chemical, structural, and dynamic conformational properties, which can only be partially mimicked by W-C base pairs. Here, I characterize the identities and tasks of six mutant G-U and A-C mispairs in Escherichia coli tRNA~(Gly) using genetic and bioinformatic tools and show that mispairs are significantly more important for aminoacylation and translation thzan previously realized. Mispairs boost aminoacylation and translation primarily because they activate tRNA by means of their conformational flexibility. The statistical preservation of the six mutant mispair sites across tRNA~(Gly) in many organisms points to a fundamental structure-function signature within tRNA~(Gly) with possible analogous missions in other RNAs.
机译：转移RNA（tRNA）分子通常包含由Watson-Crick（W-C）碱基对和偶发错误配对（如G-U和A-C）组成的四个茎。后者的配对是在几乎每类RNA中发现的RNA二级结构的基本单位，并且与W-C对几乎同构。因此，它们通常替代G-C或A-U碱基对。错配对还具有独特的化学，结构和动态构象特性，只能部分被W-C碱基对模仿。在这里，我使用遗传和生物信息学工具表征了大肠杆菌tRNA〜（Gly）中6个突变的G-U和A-C错配对的身份和任务，并表明错配对对于先前实现的氨基酰化和翻译而言更为重要。失配对促进氨基酰化和翻译，主要是因为它们通过构象柔韧性激活tRNA。在许多生物中，横跨tRNA_（Gly）的六个突变错配位点的统计保存表明，tRNA_（Gly）中具有基本的结构功能特征，而其他RNA可能具有类似的功能。

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