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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Expression of Caenorhabditis elegans neurotransmitter receptors and ion channels in Xenopus oocytes
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Expression of Caenorhabditis elegans neurotransmitter receptors and ion channels in Xenopus oocytes

机译：秀丽隐杆线虫神经递质受体和离子通道在非洲爪蟾卵母细胞中的表达

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摘要

Injection of Caenorhabditis elegans polyA RNA into Xenopus laevis oocytes led to the expression of neurotransmitter receptors that generated some unique responses, including ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors as well as receptors that coupled to G proteins, such as those to octopamine, norepinephrine, and angiotensin, which activated the oocyte's own phosphatidylinositol system and calcium-gated chloride channels. The oocytes also expressed chloride-conducting glutamate receptors, muscarinic acetylcholine receptors, and voltage-operated calcium channels. Unexpectedly, serotonin (5-hydroxytryptamine), dopamine, GABA, and kainate did not generate ionic currents, suggesting that the corresponding receptors were not expressed or were not functional in the oocytes. The use of X. laevis oocytes for expressing worm RNA demonstrates that there are many molecular components whose role remains to be clarified in the nematode. Among them are the nature of the endogenous agonists for the octopamine and angiotensin receptors and the subunits that compose the ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors and the norepinephrine receptors that couple to the phosphoinositide cascade.
机译：将秀丽隐杆线虫polyA RNA注射到非洲爪蟾卵母细胞中导致神经递质受体的表达，该受体产生一些独特的反应，包括离子型α-氨基-3-羟基-5-甲基-5-异-4-唑基丙酸受体以及与G偶联的受体蛋白质，例如对章鱼胺，去甲肾上腺素和血管紧张素的蛋白质，它们激活了卵母细胞自身的磷脂酰肌醇系统和钙门控氯化物通道。卵母细胞还表达了传导氯的谷氨酸受体，毒蕈碱乙酰胆碱受体和电压操纵的钙通道。出乎意料的是，5-羟色胺（5-羟色胺），多巴胺，GABA和海藻酸盐不会产生离子电流，表明相应的受体在卵母细胞中不表达或不起作用。 X. laevis卵母细胞表达蠕虫RNA的使用表明，有许多分子成分的作用在线虫中仍有待阐明。其中包括章鱼胺和血管紧张素受体的内源性激动剂的性质，以及组成离子型α-氨基-3-羟基-5-甲基-5-异-4-唑基丙酸受体的亚基和与磷酸肌醇级联的去甲肾上腺素受体。

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