...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The influence of chromosome flexibility on chromosome transport during anaphase A
【24h】

The influence of chromosome flexibility on chromosome transport during anaphase A

机译：染色体柔性对后期A染色体运输的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The role of protein flexibility in molecular motor function has previously been studied by considering a Brownian ratchet motor that is connected to its cargo by an elastic spring, with the result that the average velocity of the motor/cargo system is increased by reducing the stiffness of the linkage. Here, we extend this investigation to the case of chromosome transport during anaphase A, in which the relevant flexibility is not primarily in the motor/cargo linkage but rather in the cargo itself, i.e., in the chromosome. We model the motor mechanism as an imperfect Brownian ratchet with a built-in opposing load and the chromosome as a collection of discrete segments linked by an elastic energy function that discretizes the potential energy of an elastic rod. Thermal fluctuations are produced in the model by random forces, as in Brownian dynamics. All of the parameters that characterize the chromosome are known or can be estimated from experimental data, as can all but one of the motor parameters, which is adjusted to give the correct transport velocity of normal-length chromosomes. With the parameters so determined, we then reproduce the experimental finding of Nicklas [Nicklas, R. B. (1965) J. Cell Biol. 25, 119-135] that chromosome speed is essentially independent of chromosome length, even though our model contains no "velocity governor." We find instead that this effect is a consequence of chromosome flexibility, as it disappears when stiffer than normal chromosomes are considered.
机译：以前已经通过考虑通过弹性弹簧连接到其货物的布朗棘轮式电动机研究了蛋白质柔性在分子电动机功能中的作用，结果是通过降低电动机的刚性来提高电动机/货物系统的平均速度。链接。在这里，我们将这项研究扩展到后期A期间染色体运输的情况，在这种情况下，相关的灵活性主要不是在马达/货物链接中，而是在货物本身即染色体中。我们将运动机制建模为具有内置反向负载的不完善的布朗棘轮，并将染色体建模为通过离散弹性杆势能的弹性能量函数链接的离散段的集合。像布朗动力学一样，热力波动是由随机力在模型中产生的。表征染色体的所有参数都是已知的，或者可以从实验数据中估计出来，除了运动参数之一以外的所有参数都可以，可以调整这些运动参数以提供正常长度染色体的正确转运速度。利用如此确定的参数，我们然后再现了Nicklas的实验发现[Nicklas，R. B.（1965）J. Cell Biol。Chem。 25，119-135]，即使我们的模型不包含“速度调节器”，染色体速度也基本上与染色体长度无关。取而代之的是，我们发现这种影响是染色体柔韧性的结果，因为当考虑比正常染色体更坚硬时，这种影响就会消失。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号