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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Hyperaccumulation of arsenic in the shoots of Arabidopsis silenced for arsenate reductase (ACR2)
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Hyperaccumulation of arsenic in the shoots of Arabidopsis silenced for arsenate reductase (ACR2)

机译：砷还原酶（ACR2）沉默的拟南芥芽中砷的过度积累

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摘要

Endogenous plant arsenate reductase (ACR) activity converts arsenate to arsenite in roots, immobilizing arsenic below ground. By blocking this activity, we hoped to construct plants that would mobilize more arsenate aboveground. We have identified a single gene in the Arabidopsis thaliana genome, ACR2, with moderate sequence homology to yeast arsenate reductase. Expression of ACR2 cDNA in Escherichia coli complemented the arsenate-resistant and arsenate-sensitive phenotypes of various bacterial ars operon mutants. RNA interference reduced ACR2 protein expression in Arabidopsis to as low as 2% of wild-type levels. The various knockdown plant lines were more sensitive to high concentrations of arsenate, but not arsenite, than wild type. The knockdown lines accumulated 10- to 16-fold more arsenic in shoots (350-500 ppm) and retained less arsenic in roots than wild type, when grown on arsenate medium with < 8 ppm arsenic. Reducing expression of ACR2 homologs in tree, shrub, and grass species should play a vital role in the phytoremediation of environmental arsenic contamination.
机译：内源性植物砷还原酶（ACR）活性可将根中的砷转化为亚砷酸盐，从而将砷固定在地下。通过阻止这项活动，我们希望建造能够动员地上更多砷的植物。我们已经确定了拟南芥基因组中的单个基因，ACR2，与酵母砷酸还原酶具有中等序列同源性。 ACR2 cDNA在大肠杆菌中的表达补充了各种细菌ars操纵子突变体的抗砷酸盐和抗砷酸盐的表型。 RNA干扰使拟南芥中的ACR2蛋白表达降低至野生型水平的2％。各种击倒植物系对高浓度的砷酸盐而不是亚砷酸盐比野生型更敏感。当在砷含量小于8 ppm的砷酸盐培养基上生长时，击倒品系（350-500 ppm）在芽中积累的砷增加10至16倍，并且在根部保留的砷较少。减少树木，灌木和草种中ACR2同源物的表达在环境砷污染的植物修复中应发挥重要作用。

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