Spatiotemporal control of spindle midzone formation by PRC1 in human cells

Zhu CJ; Lau E; Schwarzenbacher R; Bossy-Wetzel E; Jiang W

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Spatiotemporal control of spindle midzone formation by PRC1 in human cells

机译：PRC1在人类细胞中对纺锤体中区形成的时空控制

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摘要

We have examined the role of PRC1, a micizone-associated, microtubule bundling, Cdk substrate protein, in regulating the spatio-temporal formation of the midzone in HeLa cells. Cdk-mediated phosphorylation of PRC1 in early mitosis holds PRC1 in an inactive monomeric state. During the metaphase-to-anaphase transition, PRC1 is dephosphorylated, promoting PRC1 oligomerization. Using time-lapse video microscopy, RNA interference, 3D immunofluorescence reconstruction imaging, and rescue experiments, we demonstrate that the dephosphorylated form of PRC1 is essential for bundling antiparallel, nonkinetochore, interdigitating microtubules to establish the midzone that is necessary for cytokinesis. Our results thus indicate that PRC1 is an essential factor in controlling the spatio-temporal formation of the midzone in human cells.

机译：我们已经检查了PRC1，一种与miczone关联的微管束，Cdk底物蛋白，在调节HeLa细胞中中间带的时空形成中的作用。 Cdk介导的有丝分裂早期PRC1的磷酸化使PRC1处于非活性单体状态。在中期到后期的过渡过程中，PRC1被去磷酸化，从而促进PRC1的低聚。使用延时视频显微镜，RNA干扰，3D免疫荧光重建成像和救援实验，我们证明PRC1的去磷酸化形式对于捆绑反平行，非动粒，指状微管建立细胞分裂所必需的中间区至关重要。因此，我们的结果表明，PRC1是控制人细胞中区时空形成的重要因素。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2006年第16期|p. 6196-6201|共6页
作者
Zhu CJ; Lau E; Schwarzenbacher R; Bossy-Wetzel E; Jiang W;
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作者单位

Burnham Inst Med Res, La Jolla, CA 92037 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Cdk phosphorylation; mitosis / cytokinesis; microtubule-associated proteins; microtubule bundling; FISSION YEAST; IN-VIVO; CYTOKINESIS; COMPLETION; INACTIVATION; METAPHASE; DIVISION; BINDING; MATRIX; ROLES;

机译：Cdk磷酸化;有丝分裂/胞质分裂;微管相关蛋白;微管束;裂变酵母;体内;细胞动力学;完成;失活;代谢;分裂;结合;基质;作用;

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1. Cell cycle-dependent translocation of PRC1 on the spindle by Kif4 is essential for midzone formation and cytokinesis. [J] . Zhu C, Jiang W Proceedings of the National Academy of Sciences of the United States of America . 2005,第2期

机译：Kif4在纺锤体上对PRC1的细胞周期依赖性转位对于中间区形成和胞质分裂至关重要。
2. Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells [J] . Changjun Zhu, Ella Bossy-Wetzel, Wei Jiang The biochemical journal . 2005,第2期

机译：INCENP将MKLP1募集到纺锤体中区/中体对于中体形成和完成人体细胞的胞质分裂至关重要
3. Recruitment of MKLP1 to the spindle midzone/midbody by INCENP is essential for midbody formation and completion of cytokinesis in human cells [J] . Changjun Zhu, Ella Bossy-Wetzel, Wei Jiang The biochemical journal . 2005,第2期

机译：INCENP将MKLP1募集到纺锤体中区/中体对于中体形成和完成人体细胞的胞质分裂至关重要
4. Spatiotemporal Control of Human Stem Cell Derived Tissue Spheroids [C] . Jerome Karpiak, Neil Chi, Adah Almutairi . 2012

机译：人干细胞衍生组织球体的时空控制。
5. Role of spindle motors and their regulation on chromosomal movement and spindle formation during cell division. [D] . Knight, Valeriya Vorozhko. 2011

机译：主轴电机的作用及其对细胞分裂过程中染色体运动和主轴形成的调控。
6. Spatiotemporal control of spindle midzone formation by PRC1 in human cells [O] . Changjun Zhu, Eric Lau, Robert Schwarzenbacher, 2006

机译：PRC1在人类细胞中对纺锤体中区形成的时空控制
7. Spatiotemporal control of spindle midzone formation by PRC1 in human cells [O] . Zhu, Changjun, Lau, Eric, Schwarzenbacher, Robert, 2006

机译：PRC1在人类细胞中对纺锤体中区形成的时空控制

Spatiotemporal control of spindle midzone formation by PRC1 in human cells

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