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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases
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The crystal structure of SdsA1, an alkylsulfatase from Pseudomonas aeruginosa, defines a third class of sulfatases

机译：SdsA1（铜绿假单胞菌的烷基硫酸酯酶）的晶体结构定义了第三类硫酸酯酶

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摘要

Pseudomonas aeruginosa is both a ubiquitous environmental bacterium and an opportunistic human pathogen. A remarkable metabolic versatility allows it to occupy a multitude of ecological niches, including wastewater treatment plants and such hostile environments as the human respiratory tract. P. aeruginosa is able to degrade and metabolize biocidic SDS, the detergent of most commercial personal hygiene products. We identify SclsAll of A aeruginosa as a secreted SIDS hydrolase that allows the bacterium to use primary sulfates such as SDS as a sole carbon or sulfur source. Homologues of SdsA1 are found in many pathogenic and some nonpathogenic bacteria. The crystal structure of SdsA1 reveals three distinct domains. The N-terminal catalytic domain with a binuclear Zn2+ cluster is a distinct member of the metallo-beta-lactamase fold family, the central dimerization domain ensures resistance to high concentrations of SIDS, whereas the C-terminal domain provides a hydrophobic groove, presumably to recruit long aliphatic substrates. Crystal structures of apo-SdsAl and complexes with substrate analog and products indicate an enzymatic mechanism involving a water molecule indirectly activated by the Zn2+ cluster. The enzyme SclsAl thus represents a previously undescribed class of sulfatases that allows A aeruginosa to survive and thrive under otherwise bacteriocidal conditions.
机译：铜绿假单胞菌既是普遍存在的环境细菌，又是机会性人类病原体。出色的代谢多功能性使其能够占据众多生态位，包括废水处理厂和诸如人类呼吸道等恶劣环境。铜绿假单胞菌能够降解和代谢杀菌型SDS，这是大多数商业个人卫生产品的洗涤剂。我们将铜绿假单胞菌的SclsAll鉴定为一种分泌的SIDS水解酶，该细菌可以使细菌使用伯硫酸盐（例如SDS）作为唯一的碳或硫源。在许多致病性细菌和一些非致病性细菌中发现了SdsA1的同源物。 SdsA1的晶体结构揭示了三个不同的域。具有双核Zn2 +簇的N末端催化结构域是金属β-内酰胺酶折叠家族的一个独特成员，中央二聚结构域确保了对高浓度SIDS的抵抗力，而C末端结构域提供了疏水沟，大概是募集长的脂肪族底物。 apo-SdsAl的晶体结构以及与底物类似物和产物的复合物表明了一种酶促机制，涉及被Zn2 +簇间接激活的水分子。因此，酶SclsA1代表先前未描述的一类硫酸酯酶，其使铜绿假单胞菌能够在其他杀菌条件下存活并繁衍生息。

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