...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated
【24h】

Expression profiling reveals meiotic male germ cell mRNAs that are translationally up- and down-regulated

机译：表达谱显示减数分裂的雄性生殖细胞mRNA在翻译上调和下调

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gametes rely heavily on posttranscriptional control mechanisms to regulate their differentiation. In eggs, maternal mRNAs are stored and selectively activated during development. In the male, transcription ceases during spermiogenesis, necessitating the posttranscriptional regulation of many paternal mRNAs required for spermatozoan assembly and function. To date, most of the testicular mRNAs known to be translationally regulated are initially transcribed in postmeiotic cells. Because protein synthesis occurs on polysomes and translationally inactive mRNAs are sequestered as ribonucleoproteins (RNPs), movement of mRNAs between these fractions is indicative of translational up- and down-regulation. Here, we use microarrays to analyze mRNAs in RNPs and polysomes from testis extracts of prepuberal and adult mice to characterize the translation state of individual mRNAs as spermatogenesis proceeds. Consistent with published reports, many of the translationally delayed postmeiotic mRNAs shift from the RNPs into the polysomes, establishing the validity of this approach. In addition, we detect another 742 mouse testicular transcripts that show dramatic shifts between RNPs and polysomes. One subgroup of 35 genes containing the known, translationally delayed phosphoglycerate kinase 2 (Pgk2) is initially transcribed during meiosis and is translated in later-stage cells. Another subgroup of 82 meiotically expressed genes is translationally down-regulated late in spermatogenesis. This high-throughput approach defines the changing translation patterns of populations of genes as male germ cells differentiate and identifies groups of meiotic transcripts that are translationally up- and down-regulated.
机译：配子严重依赖转录后控制机制来调节其分化。在卵中，母体mRNA被储存并在发育过程中被选择性激活。在雄性中，转录在精子发生期间停止，因此需要转录后调节精子组装和功能所需的许多父本mRNA。迄今为止，大多数已知被翻译调控的睾丸mRNA最初都在减数分裂后的细胞中转录。因为蛋白质合成发生在多核糖体上，并且翻译无活性的mRNA被隔离为核糖核蛋白（RNP），所以这些部分之间的mRNA移动表明翻译上调和下调。在这里，我们使用微阵列分析从青春期前和成年小鼠睾丸提取物中的RNP和多核糖体中的mRNA，以表征随着精子发生过程个别mRNA的翻译状态。与已发表的报道一致，许多翻译后减数分裂后的mRNA从RNP转变为多核糖体，确立了这种方法的有效性。此外，我们检测到另外742个小鼠睾丸转录本，显示RNP和多核糖体之间发生了巨大变化。包含已知翻译延迟磷酸甘油酸激酶2（Pgk2）的35个基因的一个亚组在减数分裂过程中首先被转录，并在后期细胞中被翻译。 82个减数分裂表达基因的另一个亚组在精子发生后期被翻译性下调。这种高通量方法定义了随着雄性生殖细胞的分化而改变的基因种群翻译模式，并鉴定了在翻译上调和下调的减数分裂转录物组。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号