...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor
【24h】

Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor

机译：植物细胞识别几丁质片段以通过质膜受体防御信号

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants, chitin oligosaccharides have been known to induce various defense responses in a wide range of plant cells including both monocots and dicots. To clarify the molecular machinery involved in the perception and transduction of chitin oligosaccharide elicitor, a high-affinity binding protein for this elicitor was isolated from the plasma membrane of suspension-cultured rice cells. Characterization of the purified protein, CEBiP, as well as the cloning of the corresponding gene revealed that CEBiP is actually a glycoprotein consisting of 328 amino acid residues and glycan chains. CEBiP was predicted to have a short membrane spanning domain at the C terminus. Knockdown of CEBiP gene by RNA interference resulted in the suppression of the elicitor-induced oxidative burst as well as the gene responses, showing that CEBiP plays a key role in the perception and transduction of chitin oligosaccharide elicitor in the rice cells. Structural analysis of CEBiP also indicated the presence of two LysM motifs in the extracellular portion of CEBiP. As the LysM motif has been known to exist in the putative Nod-factor receptor kinases involved in the symbiotic signaling between leguminous plants and rhizobial bacteria, the result indicates the involvement of partially homologous plasma membrane proteins both in defense and symbiotic signaling in plant cells.
机译：几丁质是真菌细胞壁的主要成分，并作为一种分子模式，用于识别动植物先天免疫系统中的潜在病原体。在植物中，已知几丁质寡糖可在包括单子叶植物和双子叶植物的多种植物细胞中诱导各种防御反应。为了阐明涉及几丁质寡糖引发剂的感知和转导的分子机制，从悬浮培养的水稻细胞的质膜中分离出该引发剂的高亲和力结合蛋白。纯化蛋白CEBiP的表征以及相应基因的克隆表明，CEBiP实际上是一种由328个氨基酸残基和聚糖链组成的糖蛋白。预计CEBiP在C末端具有短的跨膜结构域。 RNAi干扰CEBiP基因的敲除导致诱导子诱导的氧化爆发以及基因响应的抑制，表明CEBiP在水稻细胞中几丁质寡糖诱导子的感知和转导中起关键作用。 CEBiP的结构分析还表明在CEBiP的细胞外部分中存在两个LysM基序。由于已知LysM基序存在于豆科植物和根瘤菌之间的共生信号涉及的推定Nod因子受体激酶中，因此该结果表明部分同源的质膜蛋白参与了植物细胞的防御和共生信号传导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号