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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Different mitochondrial-intermembrane space proteins are released during apoptosis in a manner that is coordinately initiated but can vary in duration
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Different mitochondrial-intermembrane space proteins are released during apoptosis in a manner that is coordinately initiated but can vary in duration

机译：细胞凋亡过程中以协调启动的方式释放不同的线粒体-跨膜空间蛋白，但持续时间可能会有所不同

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摘要

The release of mitochondrial intermembrane space proteins to the cytosol is a key event during apoptosis. We used in situ fluorescent labeling of proteins tagged with a short tetracysteine-containing sequence to follow the release of Smac, Omi, adenylate kinase-2, cytochrome c, and apoptosis-inducing factor (AIF) during apoptosis and compared the release with that of cytochrome c tagged with GFP in individual cells observed overtime. We observed a caspase-independent, simultaneous release of cytochrome c, Smac, Omi, and adenylate kinase-2. Although AIF release also was caspase-independent and commenced with that of the other proteins, it proceeded much more slowly and incompletely from mitochondria, perhaps because of a requirement for a secondary event. These results suggest that these proteins are released through the same mitochondrial pore and that apoptosis may not be regulated through a selective release of individual mitochondrial proteins. The timing and extent of AIF release makes it unlikely that it is involved in the induction of apoptosis, either upstream or downstream of mitochondrial outer membrane permeabilization.
机译：线粒体膜间空间蛋白释放到细胞质中是细胞凋亡过程中的关键事件。我们使用原位荧光标记具有短四半胱氨酸序列的蛋白质来追踪Smac，Omi，腺苷酸激酶2，细胞色素c和凋亡诱导因子（AIF）在细胞凋亡过程中的释放，并将其与长时间观察到的单个细胞中用GFP标记的细胞色素c。我们观察到caspase独立，同时释放细胞色素c，Smac，Omi和腺苷酸激酶2。尽管AIF的释放也与caspase无关，并且以其他蛋白质开始释放，但它可能从线粒体开始缓慢而不完全地进行，可能是由于需要继发性事件。这些结果表明，这些蛋白质是通过相同的线粒体孔释放的，并且凋亡可能不会通过选择性释放单个线粒体蛋白来调节。 AIF释放的时间和程度使其不可能参与线粒体外膜通透性上游或下游的细胞凋亡诱导。

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