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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in Drosophila
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Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in Drosophila

机译：神经毒性蛋白表达揭示了果蝇昼夜节律与交配行为之间的联系

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摘要

To investigate the functions of circadian neurons, we added two strategies to the standard Drosophila behavioral genetics repertoire. The first was to express a polyglutamine-expanded neurotoxic protein (MJDtr78Q; MJD, Machado-Joseph disease) in the major timeless (tim)-expressing cells of the adult brain. These Tim-MJD flies were viable, in contrast to the use of cell-death gene expression for tim neuron inactivation. Moreover, they were more arrhythmic than flies expressing other neurotoxins and had low but detectable tim mRNA levels. The second extended standard microarray technology from fly heads to dissected fly brains. By combining the two approaches, we identified a population of Tim-MJD-affected mRNAs. Some had been previously identified as sex-specific and relevant to courtship, including mRNAs localized to brain-proximal fat-body tissue and brain courtship centers. Finally, we found a decrease in the number of neurons that expressed male-specific forms of the fruitless protein in the laterodorsal region of the brain. The decrease was not a consequence of toxic protein expression within these specialized cells but a likely effect of communication with neighboring TIM-expressing neurons. The data suggest a functional interaction between adjacent circadian and mating circuits within the fly brain, as well as an interaction between circadian circuits and brain-proximal fat body.
机译：为了研究昼夜节律神经元的功能，我们在果蝇行为遗传学标准库中添加了两种策略。第一个是在成年大脑主要的永恒（tim）表达细胞中表达一种聚谷氨酰胺扩展的神经毒性蛋白（MJDtr78Q； MJD，Machado-Joseph病）。这些Tim-MJD苍蝇是可行的，与使用细胞死亡基因表达进行Tim神经元失活相反。此外，它们比表达其他神经毒素的蝇类更不规律，并且tim mRNA水平低但可检测。第二种将标准微阵列技术从蝇头扩展到解剖的蝇头。通过结合这两种方法，我们确定了受Tim-MJD影响的mRNA群体。某些先前已被确定为具有性别特异性且与求爱相关，包括位于大脑附近脂肪组织和脑部求爱中心的mRNA。最后，我们发现在大脑的后嗅区域表达无特异性蛋白质的雄性特异性形式的神经元数量减少。减少不是这些专门细胞中毒性蛋白表达的结果，而是与邻近的表达TIM的神经元通讯的可能影响。数据表明，苍蝇脑内相邻的昼夜节律与交配回路之间存在功能相互作用，以及昼夜节律与大脑近端脂肪体之间存在相互作用。

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