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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The herpesvirus glycoproteins B and H·L are sequentially recruited to the receptor-bound gD to effect membrane fusion at virus entry
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The herpesvirus glycoproteins B and H·L are sequentially recruited to the receptor-bound gD to effect membrane fusion at virus entry

机译：疱疹病毒糖蛋白B和H·L依次募集到受体结合的gD，以在病毒进入时实现膜融合

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摘要

Four glycoproteins (gD, gB, gH, and gL) are required for herpes simplex virus entry into the cell or for cell-cell fusion in transfected cells. gD serves as the receptor-binding glycoprotein and as the trigger of fusion; the other three execute fusion between the viral envelope and the plasma and endocytic membranes or the membranes of adjacent cells and are highly conserved among members of the herpesvirus family. Details of the interaction of gD with gB, gH, and gL were not known. Here, we report that the four glycoproteins assemble into a complex initiated by the interaction of gD with its cellular receptor. gB is recruited to the gD-receptor complex next, even in the absence of gH·gL. gH·gL is recruited next, but only to the receptor-gD-gB ensemble. A complex with the composition receptor-gD-gB-gH·gL is assembled transiently with a life span of 15-30 min in cells exposed to virus but can also be found in infected cells and in cells committed to form polykaryo-cytes after transfection of the glycoprotein quartet. The results indicate that the complex assembly is a critical step in the process of virus entry and fusion, and that no viral protein other than those that participate in the complex itself is required for complex assembly. These findings imply critical protein-protein interactions among the quartet as herpes simplex virions enter the cells and at cell-cell fusion, define a specific order of recruitment, and place gH-gL as the last link in the process of glycoprotein recruitment to the complex.
机译：单纯疱疹病毒进入细胞或转染细胞中的细胞融合需要四种糖蛋白（gD，gB，gH和gL）。 gD充当受体结合糖蛋白，并引发融合；其他三个在病毒包膜与质膜和内吞膜或相邻细胞膜之间进行融合，并且在疱疹病毒家族成员中高度保守。 gD与gB，gH和gL相互作用的细节尚不清楚。在这里，我们报道了四种糖蛋白组装成由gD及其细胞受体相互作用而引发的复合物。接下来，即使没有gH·gL，gB也被募集到gD受体复合物中。接下来募集gH·gL，但仅在受体-gD-gB集合中募集。具有组成成分的受体-gD-gB-gH·gL的复合物在暴露于病毒的细胞中以15-30分钟的寿命短暂组装，但也可以在感染的细胞和转染后致力于形成多核细胞的细胞中发现糖蛋白四重奏。结果表明，复杂的装配是病毒进入和融合过程中的关键步骤，复杂装配不需要任何病毒蛋白（参与复合物本身的除外）。这些发现暗示当单纯疱疹病毒粒子进入细胞并在细胞-细胞融合时，四元组之间至关重要的蛋白质-蛋白质相互作用，定义了特定的募集顺序，并将gH-gL作为糖蛋白募集到复合物中的最后一个环节。

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