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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Independent and simultaneous translocation of two substrates by a nucleotide sugar transporter
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Independent and simultaneous translocation of two substrates by a nucleotide sugar transporter

机译：核苷酸糖转运蛋白可同时独立转运两种底物

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摘要

Nucleotide sugar transporters play an essential role in protein and lipid glycosylation, and mutations can result in developmental phenotypes. We have characterized a transporter of UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine encoded by the Caenorhabditis elegans gene C03H15.2. Surprisingly, translocation of these substrates occurs in an independent and simultaneous manner that is neither a competitive nor a symport transport. Incubations of Golgi apparatus vesicles of Saccharomyces cerevisiae expressing C03H5.2 protein with these nucleotide sugars labeled with H-3 and C-14 in their sugars showed that both substrates enter the lumen to the same extent, whether or not they are incubated alone or in the presence of a 10-fold excess of the other nucleotide sugar. Vesicles containing a deletion mutant of the C03H15.2 protein transport UDP-N-acetylglucosamine at rates comparable with that of wild-type transporter, whereas transport of UDP-N-acetylgalactosamine was decreased by 85-90%, resulting in an asymmetrical loss of substrate transport.
机译：核苷酸糖转运蛋白在蛋白质和脂质糖基化中起重要作用，而突变可导致发育表型。我们已经表征了秀丽隐杆线虫基因C03H15.2编码的UDP-N-乙酰氨基葡糖和UDP-N-乙酰半乳糖胺的转运蛋白。令人惊讶地，这些底物的易位以独立和同时的方式发生，既不是竞争性运输也不是共担运输。用糖中带有H-3和C-14标记的核苷酸糖对表达C03H5.2蛋白的酿酒酵母的高尔基体小泡进行培养，表明无论是单独培养还是在单独培养中，两种底物均以相同的程度进入内腔。存在10倍过量的其他核苷酸糖。含有C03H15.2蛋白缺失突变体的囊泡以与野生型转运蛋白相当的速率转运UDP-N-乙酰氨基葡萄糖，而UDP-N-乙酰半乳糖胺的转运减少了85-90％，导致不对称损失。底物运输。

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