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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of a large noncoding RNA in extremophilic eubacteria
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Identification of a large noncoding RNA in extremophilic eubacteria

机译:鉴定极端嗜酸性真细菌中的大型非编码RNA

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摘要

We have discovered a large and highly conserved RNA motif that typically resides in a noncoding section of a multigene messenger RNA in extremophilic Gram-positive eubacteria. RNAs of this class adopt an ornate secondary structure, are large compared with most other noncoding RNAs, and have been identified only in certain extremophilic bacteria. These ornate, large, extremophilic (OLE) RNAs have a length of approximate to 610 nucleoticles, and the 35 representatives examined exhibit extraordinary conservation of nucleotide sequence and base pairing. Structural probing of the OLE RNA from Bacillus halodurans corroborates a complex secondary structure model predicted from comparative sequence analysis. The patterns of structural conservation, and its unique phylogenetic distribution, suggest that OLE RNA carries out a complex and critical function only in certain extremophilic bacteria.
机译:我们发现了一个大型且高度保守的RNA基序,该基序通常位于极端的革兰氏阳性真细菌中的多基因信使RNA的非编码部分。此类RNA具有华丽的二级结构,与大多数其他非编码RNA相比,较大,并且仅在某些极端嗜热细菌中得到鉴定。这些华丽的,大的,极端的(OLE)RNA的长度大约为610个核苷酸,所检查的35位代表表现出核苷酸序列和碱基配对的非凡保守性。来自嗜盐芽孢杆菌的OLE RNA的结构探测证实了根据比较序列分析预测的复杂二级结构模型。结构保守性的模式及其独特的系统发育分布表明,OLE RNA仅在某些极端的细菌中才具有复杂而关键的功能。

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