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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Localized proton microcircuits at the biological membrane-water interface
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Localized proton microcircuits at the biological membrane-water interface

机译：生物膜-水界面处的局部质子微电路

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摘要

Cellular processes such as nerve conduction, energy metabolism, and import of nutrients into cells all depend on transport of ions across biological membranes through specialized membrane-spanning proteins. Understanding these processes at a molecular level requires mechanistic insights into the interaction between these proteins and the membrane itself. To explore the role of the membrane in ion translocation we used an approach based on fluorescence correlation spectroscopy. Specifically, we investigated exchange of protons between the water phase and the membrane surface, as well as diffusion of protons along membrane surfaces, at a single-molecule level. We show that the lipid head groups collectively act as a proton-collecting antenna, dramatically accelerating proton uptake from water to a membrane-anchored proton acceptor. Furthermore, the results show that proton transfer along the surface can be significantly faster than that between the lipid head groups and the surrounding water phase. Thus, ion translocation across membranes and between the different membrane protein components is a complex interplay between the proteins and the membrane itself, where the membrane acts as a proton-conducting link between membrane-spanning proton transporters.
机译：神经传导，能量代谢和营养物质进入细胞等细胞过程都依赖于离子通过跨膜的蛋白质通过专门的跨膜蛋白的运输。在分子水平上理解这些过程需要对这些蛋白质与膜本身之间相互作用的机理进行深入研究。为了探索膜在离子转运中的作用，我们使用了一种基于荧光相关光谱的方法。具体而言，我们研究了在单分子水平上水相与膜表面之间质子的交换以及质子沿膜表面的扩散。我们显示脂质头基团集体充当质子收集天线，大大加速了质子从水中向膜锚定质子受体的吸收。此外，结果表明，质子沿表面的传输可能比脂质头基团与周围水相之间的传输快得多。因此，跨膜以及不同膜蛋白成分之间的离子易位是蛋白与膜本身之间的复杂相互作用，其中膜充当跨膜质子转运蛋白之间的质子传导连接。

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