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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of a nitrogenase FeMo cofactor precursor on NifEN complex
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Identification of a nitrogenase FeMo cofactor precursor on NifEN complex

机译：NifEN配合物上的固氮酶FeMo辅因子前体的鉴定

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摘要

The biosynthesis of the FeMo cofactor (FeMoco) of Azotobacter vinelandii nitrogenase presumably starts with the production of its Fe/S core by NifB (the nifB gene product). This core is subsequently processed on the α_2β_2 tetrameric NifEN complex (formed by the nifE and nifN gene products). In this article, we identify a NifEN-bound FeMoco precursor form that can be converted to fully assembled FeMoco in a so-called FeMoco-maturation assay containing only purified components. We also establish that only molybdate, homocitrate, MgATP, and Fe protein are essential for FeMoco maturation. The FeMoco-maturation assay described here will further address the remaining questions related to the assembly mechanism of the ever-intriguing FeMoco.
机译：葡萄固氮菌固氮酶的FeMo辅因子（FeMoco）的生物合成大概始于NifB（nifB基因产物）产生的Fe / S核心。随后在α_2β_2四聚体NifEN复合物（由nifE和nifN基因产物形成）上加工该核心。在本文中，我们确定了与NifEN结合的FeMoco前驱体形式，可以在仅包含纯化成分的所谓FeMoco成熟试验中将其转化为完全组装的FeMoco。我们还确定，只有钼酸盐，纯柠檬酸盐，MgATP和Fe蛋白对于FeMoco成熟至关重要。本文所述的FeMoco成熟度测定法将进一步解决与有趣的FeMoco组装机制有关的其余问题。

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