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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by single-pair fluorescence resonance energy transfer
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Fast, long-range, reversible conformational fluctuations in nucleosomes revealed by single-pair fluorescence resonance energy transfer

机译：单对荧光共振能量转移揭示核小体中快速，远距离，可逆的构象波动

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摘要

The nucleosome core particle, the basic repeated structure in chromatin fibers, consists of an octamer of eight core histone molecules, organized as dimers (H2A/H2B) and tetramers [(H3/H4)_2] around which DNA wraps tightly in almost two left-handed turns. The nucleosome has to undergo certain conformational changes to allow processes that need access to the DNA template to occur. By single-pair fluorescence resonance energy transfer, we demonstrate fast, long-range, reversible conformational fluctuations in nucleosomes between two states: fully folded (closed), with the DNA wrapped around the histone core, or open, with the DNA significantly unraveled from the histone octamer. The brief excursions into an extended open state may create windows of opportunity for protein factors involved in DNA transactions to bind to or translocate along the DNA.
机译：核小体核心颗粒是染色质纤维中的基本重复结构，由八个核心组蛋白分子的八聚体组成，组织为二聚体（H2A / H2B）和四聚体[（H3 / H4）_2]，DNA几乎被两个分子紧紧包裹手转弯。核小体必须经历某些构象变化，以允许需要访问DNA模板的过程发生。通过单对荧光共振能量转移，我们证明了核小体在以下两种状态之间的快速，长距离，可逆构象波动：完全折叠（闭合），DNA包裹在组蛋白核心上，或打开，DNA显着脱离组蛋白八聚体。短暂的进入延长的开放状态可能会为参与DNA交易的蛋白质因子结合或沿着DNA转移创造机会。

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