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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The eukaryotic P loop NTPase Nbp35: An essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery
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The eukaryotic P loop NTPase Nbp35: An essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery

机译：真核P环NTPase Nbp35：胞质和核铁硫蛋白组装机械的重要组成部分

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摘要

Soluble P loop NTPases represent a large protein family and are involved in diverse cellular functions. Here, we functionally characterized the first member of the Mrp Nbp35 subbranch of this family, the essential Nbp35p of Saccharomyces cerevisiae. The protein resides in the cytosol and nucleus and carries an Fe/S cluster at its N terminus. Assembly of the Fe/S cluster requires the mitochondrial Fe/S cluster (ISC)-assembly and -export machineries. Depletion of Nbp35p strongly impairs the activity of the cytosolic Fe/S protein, isopropylmalate isomerase (Leu1p), whereas mitochondrial Fe/S enzymes are unaffected. Moreover, defects in the de novo maturation of various cytosolic and nuclear Fe/S proteins were observed in the absence of Nbp35p, demonstrating the functional involvement of Nbp35p in the biogenesis of extramito-chondrial Fe/S proteins. Furthermore, Nbp35p genetically interacts with the closely similar P loop NTPase, Cfd1p, and the hydroge-nase-like Nar1p, both of which were recently shown to perform a crucial function in cytosolic and nuclear Fe/S protein biogenesis. Hence, our study suggests that eukaryotic Nbp35 NTPases function in Fe/S protein maturation. The findings provide strong evidence for the existence of a highly conserved and essential machinery dedicated to assembling cytosolic and nuclear Fe/S proteins.
机译：可溶性P环NTPase代表一个大蛋白家族，并参与多种细胞功能。在这里，我们在功能上表征了该家族的Mrp Nbp35亚科的第一个成员，即酿酒酵母的必需Nbp35p。该蛋白质位于细胞质和细胞核中，并在其N末端带有Fe / S簇。 Fe / S团簇的组装需要线粒体Fe / S团簇（ISC）组装和出口。 Nbp35p的耗竭强烈削弱了胞质Fe / S蛋白，苹果酸异丙基丙二酸异构酶（Leu1p）的活性，而线粒体Fe / S酶则不受影响。此外，在不存在Nbp35p的情况下，观察到了各种胞质和核Fe / S蛋白从头成熟的缺陷，表明Nbp35p在膜外软骨Fe / S蛋白的生物发生中的功能参与。此外，Nbp35p在遗传上与紧密相似的P环NTPase，Cfd1p和类似于水解酶的Nar1p相互作用，最近两者都显示出在胞质和核Fe / S蛋白生物发生中起关键作用。因此，我们的研究表明，真核Nbp35 NTPases在Fe / S蛋白成熟中起作用。这些发现为存在高度保守的，必不可少的，专门用于组装胞质和核Fe / S蛋白的机制提供了有力的证据。

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