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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Quantitative kinetic analysis of the bacteriophage λ genetic network
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Quantitative kinetic analysis of the bacteriophage λ genetic network

机译：噬菌体λ遗传网络的定量动力学分析

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摘要

The lysis-lysogeny decision of bacteriophage λ has been a paradigm for a developmental genetic network, which is composed of interlocked positive and negative feedback loops. This genetic network is capable of responding to environmental signals and to the number of infecting phages. An interplay between CⅠ and Cro functions suggested a bistable switch model for the lysis-lysogeny decision. Here, we present a real-time picture of the execution of lytic and lysogenic pathways with unprecedented temporal resolution. We monitor, in vivo, both the level and function of the CⅡ and Q gene regulators. These activators are cotranscribed yet control opposite developmental pathways. Conditions that favor the lysogenic response show severe delay and down-regulation of Q activity, in both CⅡ-dependent and CⅡ-independent ways. Whereas CⅡ activity correlates with its protein level, Q shows a pronounced threshold before its function is observed. Our quantitative analyses suggest that by regulating CⅡ and CⅢ, Cro plays a key role in the ability of the λ genetic network to sense the difference between one and more than one phage particles infecting a cell. Thus, our results provide an improved framework to explain the longstanding puzzle of the decision process.
机译：噬菌体λ的裂解-溶原决定是发展遗传网络的范例，该遗传网络由互锁的正反馈回路和负反馈回路组成。这种遗传网络能够响应环境信号和感染噬菌体的数量。 CⅠ和Cro功能之间的相互作用为裂解-溶原性决定提供了一个双稳态转换模型。在这里，我们以前所未有的时间分辨率呈现了裂解和溶源途径的实时执行情况。我们在体内监测CⅡ和Q基因调节剂的水平和功能。这些激活剂是共转录的，但控制相反的发育途径。支持溶原反应的条件以CⅡ依赖性和CⅡ依赖性两种方式表现出严重的延迟和Q活性的下调。 CⅡ的活性与其蛋白质水平相关，而Q在观察其功能之前显示出明显的阈值。我们的定量分析表明，通过调节CⅡ和CⅢ，Cro在λ遗传网络感知一个和一个以上噬菌体颗粒感染细胞之间的差异的能力中起着关键作用。因此，我们的结果提供了一个改进的框架来解释决策过程中的长期难题。

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