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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >CcbP, a calcium-binding protein from Anabaena sp. PCC 7120, provides evidence that calcium ions regulate heterocyst differentiation
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CcbP, a calcium-binding protein from Anabaena sp. PCC 7120, provides evidence that calcium ions regulate heterocyst differentiation

机译：CcbP，一种来自鱼腥藻的钙结合蛋白。 PCC 7120提供了钙离子调节异型囊肿分化的证据

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摘要

Although it is known that calcium is a very important messenger involved in many eukaryotic cellular processes, much less is known about calcium's role in bacteria. CcbP, a Ca~(2+)-binding protein, was isolated from the heterocystous cyanobacterium Anabaena sp. PCC 7120, and the ccbPgene was cloned and inactivated. In the absence of combined nitrogen, inactivation of ccbP resulted in multiple contiguous heterocysts, whereas overexpression of ccbP suppressed heterocyst formation. Calmodulin, which is not present in Anabaena species, could also suppress heterocyst formation in both Anabaena sp. PCC 7120 and Anabaena variabilis. HetR induction upon nitrogen step-down was slow in the strain overexpress-ing ccbP. The Ca~(2+) reporter protein obelin was used to show that mature heterocysts had a high intracellular free Ca~(2+)concentration {[Ca~(2+)]_i}, and immunoblotting showed that CcbP was absent from heterocysts. A regular pattern of cells with higher [Ca~(2+)]_i was established during heterocyst differentiation before the appearance of proheterocysts. A rapid increase of [Ca~(2+)]_i could be detected 4 h after the removal of combined nitrogen, and this increase was suppressed by excessive CcbP. These results suggest that Ca~(2+) ions play very important roles in hetR induction and heterocyst differentiation.
机译：尽管已知钙是参与许多真核细胞过程的非常重要的信使，但人们对钙在细菌中的作用所知甚少。 CcbP是一种Ca〜（2+）结合蛋白，是从异囊蓝藻Anabaena sp。中分离得到的。 PCC 7120和ccbP基因被克隆并失活。在缺乏氮的情况下，ccbP的失活导致多个连续的异型囊肿，而ccbP的过表达抑制了异型囊肿的形成。钙调蛋白，这并不存在于鱼腥藻物种中，也可以抑制两个鱼腥藻中异囊藻的形成。 PCC 7120和鱼腥藻。在过表达ccbP的菌株中，降氮时对HetR的诱导很慢。用Ca〜（2+）报道蛋白obelin来显示成熟的异胞具有较高的细胞内游离Ca〜（2+）浓度{[Ca〜（2 +）] _ i}，免疫印迹表明异胞没有CcbP 。在异囊胚分化过程中，在出现前囊胚之前建立了具有较高[Ca〜（2 +）] _ i的规则细胞模式。去除结合氮后4 h，[Ca〜（2 +）] _ i迅速增加，而过量的CcbP抑制了这种增加。这些结果表明Ca〜（2+）离子在hetR诱导和异胚囊分化中起着非常重要的作用。

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