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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >How sequence defines structure: A crystallographic map of DNA structure and conformation
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How sequence defines structure: A crystallographic map of DNA structure and conformation

机译：序列如何定义结构：DNA结构和构象的晶体学图

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摘要

The fundamental question of how sequence defines conformation is explicitly answered if the structures of all possible sequences of a macromolecule are determined. We present here a crystallographic screen of all permutations of the inverted repeat DNA sequence d(CCnnnN_6N_7N_8GG), where N_6, N_7, and N_8 are any of the four naturally occurring nucleotides. At this point, 63 of the 64 possible permutations have been crystallized from a defined set of solutions. When combined with previous work, we have assembled a data set of 37 single-crystal structures from 29 of the sequences in this motif, representing three structural classes of DNA (B-DNA, A-DNA, and four-stranded Holliday junctions). This data set includes a unique set of amphimorphic sequence, those that crystallize in two different conformations and serve to bridge the three structural phases. We have thus constructed a map of DNA structures that can be walked through in single nucleotide steps. Finally, the resulting data set allows us to dissect in detail the stabilization of and conformational variations within structural classes and identify significant conformational deviations within a particular structural class that result from sequence rather than crystal or crystallization effects.
机译：如果确定了大分子的所有可能序列的结构，则将明确回答有关序列如何定义构象的基本问题。我们在这里展示了反向重复DNA序列d（CCnnnN_6N_7N_8GG）的所有排列的晶体学筛查，其中N_6，N_7和N_8是四个天然核苷酸中的任何一个。至此，已经从一组确定的解决方案中确定了64种可能排列中的63种。与以前的工作结合时，我们从该主题的29个序列中组装了37个单晶结构的数据集，代表三种DNA结构类型（B-DNA，A-DNA和四链霍利迪结）。该数据集包括一组独特的两性序列，它们以两种不同的构象结晶并桥接三个结构相。因此，我们已经构建了可以在单核苷酸步骤中通过的DNA结构图。最后，所得的数据集使我们能够详细剖析结构类别内的稳定性和构象变异，并确定特定结构类别内由序列而不是晶体或结晶效应导致的显着构象偏差。

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