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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
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The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

机译：elongata突变体在植物中鉴定出功能性Elongator复合物，该复合物在器官生长过程中对细胞增殖起作用

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摘要

The key enzyme for transcription of protein-encoding genes in eukaryotes is RNA polymerase II (RNAPII). The recruitment of this enzyme during transcription initiation and its passage along the template during transcription elongation is regulated through the association and dissociation of several complexes. Elongator is a histone acetyl transferase complex, consisting of six subunits (ELP1-ELP6), that copurifies with the elongating RNAPII in yeast and humans. We demonstrate that point mutations in three Ara-bidopsis thaliana genes, encoding homologs of the yeast Elongator subunits ELP1, ELP3 (histone acetyl transferase), and ELP4 are responsible for the phenotypes of the elongata2 (e/o2), elo3, and elo1 mutants, respectively. The elo mutants are characterized by narrow leaves and reduced root growth that results from a decreased cell division rate. Morphological and molecular phenotypes show that the ELONGATA (ELO) genes function in the same biological process and the epistatic interactions between the ELO genes can be explained by the model of complex formation in yeast. Furthermore, the plant Elongator complex is genetically positioned in the process of RNAPII-mediated transcription downstream of Mediator. Our data indicate that the Elongator complex is evolutionarily conserved in structure and function but reveal that the mechanism by which it stimulates cell proliferation is different in yeast and plants.
机译：真核生物中蛋白质编码基因转录的关键酶是RNA聚合酶II（RNAPII）。该酶在转录起始过程中的募集及其在转录延伸过程中沿模板的传递通过几种复合物的缔合和解离来调节。延伸子是一种组蛋白乙酰基转移酶复合物，由六个亚基（ELP1-ELP6）组成，可与酵母和人类中的延伸RNAPII共纯化。我们证明点突变在三个Ara-bidopsis thaliana基因，编码酵母延伸子亚基ELP1，ELP3（组蛋白乙酰转移酶）和ELP4的同源物负责elongata2（e / o2），elo3和elo1突变体的表型。 ， 分别。 elo突变体的特征是叶子狭窄，根系生长减少，这是由于细胞分裂速率降低所致。形态和分子表型表明，ELONGATA（ELO）基因在相同的生物学过程中起作用，并且ELO基因之间的上位相互作用可以通过酵母中复合物的形成模型来解释。此外，植物伸长剂复合物在RNAPII介导的介体转录下游过程中遗传定位。我们的数据表明，Elongator复合物在结构和功能上是进化保守的，但揭示了它在酵母和植物中刺激细胞增殖的机制是不同的。

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