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A chemical waveform synthesizer

机译:化学波形合成器

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摘要

Algorithms and methods were developed to synthesize complex chemical waveforms in open volumes by using a scanning-probe microfluidic platform. Time-dependent variations and oscillations of one or several chemical species around the scanning probe, such as formation of sine waves, damped oscillations, and generation of more complex patterns, are demonstrated. Furthermore, we show that intricate bursting and chaotic calcium oscillations found in biological microdomains can be reproduced and that a biological cell can be used as a probe to study receptor functionalities as a function of exposure to time-dependent variations of receptor activators and inhibitors. Thus, the method allows for studies of biologically important oscillatory reactions. More generally, the system allows for detailed studies of complex time-varying chemical and physical phenomena in solution or at solution/surface interfaces.
机译:通过使用扫描探针微流体平台,开发了在开放体积中合成复杂化学波形的算法和方法。证明了扫描探针周围一种或几种化学物质随时间的变化和振荡,例如正弦波的形成,阻尼振荡以及更复杂图案的生成。此外,我们表明,可以复制在生物微域中发现的复杂的爆裂和混沌钙振荡,并且可以将生物细胞用作研究受体功能的探针,这些功能是受体激活剂和抑制剂随时间变化的函数。因此,该方法允许研究生物学上重要的振荡反应。更一般而言,该系统允许对溶液中或溶液/表面界面处的复杂时变化学和物理现象进行详细研究。

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