Telomerase can act as a template- and RNA-independent terminal transferase

Lue NF; Bosoy D; Moriarty TJ; Autexier C; Altman B; Leng SY

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Telomerase can act as a template- and RNA-independent terminal transferase

机译：端粒酶可以充当独立于模板和RNA的末端转移酶

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Telomerase is a special reverse transcriptase that extends one strand of the telomere repeat by using a template embedded in an RNA subunit. Like other polymerases, telomerase is believed to use a pair of divalent metal ions (coordinated by a triad of aspartic acid residues) for catalyzing nucleotide addition. Here we show that, in the presence of manganese, both yeast and human telomerase can switch to a template- and RNA-independent mode of DNA synthesis, acting in effect as a terminal transferase. Even as a terminal transferase, yeast telomerase retains a species-dependent preference for GT-rich, telomere-like DNA on the 5' end of the substrate. The terminal transferase activity of telomerase may account for some of the hitherto unexplained effects of telomerase overexpression on cell physiology.

机译：端粒酶是一种特殊的逆转录酶，它通过使用嵌入RNA亚基的模板来延伸端粒重复序列的一条链。与其他聚合酶一样，端粒酶被认为使用一对二价金属离子（由天冬氨酸残基的三联体配位）催化核苷酸的添加。在这里，我们表明，在锰的存在下，酵母和人类端粒酶都可以切换为DNA合成的模板和RNA无关模式，实际上起末端转移酶的作用。即使作为末端转移酶，酵母端粒酶在底物的5'端仍保留了丰富的GT端粒样DNA的物种依赖性。端粒酶的末端转移酶活性可能解释了端粒酶过表达对细胞生理学的某些迄今无法解释的影响。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2005年第28期|p. 9778-9783|共6页
作者
Lue NF; Bosoy D; Moriarty TJ; Autexier C; Altman B; Leng SY;
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作者单位

Cornell Univ, Weill Med Coll, WR Hearst Microbiol Res Ctr, Dept Microbiol & Immunol, New York, NY 10021 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
reverse transcriptase; telomere; divalent metal ion; REPEAT ADDITION PROCESSIVITY; REVERSE-TRANSCRIPTASE; TETRAHYMENA-TELOMERASE; YEAST TELOMERASE; BOUNDARY DEFINITION; IN-VIVO; DNA; NUCLEOTIDE; PROTEIN; MOTIFS;

机译：逆转录酶;端粒;二价金属离子;重复添加过程;逆转录酶;四膜膜端粒酶;酵母端粒酶;边界定义;体内;DNA;核酸;蛋白质;蛋白质;

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2. A Motif in the Vertebrate Telomerase N-Terminal Linker of TERT Contributes to RNA Binding and Telomerase Activity and Processivity [J] . Michael Harkisheimer, Mark Mason, Elena Shuvaeva, Structure . 2013,第10期

机译：TERT的脊椎动物端粒酶N末端接头中的基序有助于RNA结合以及端粒酶活性和加工性。
3. The C-terminal domain of Tetrahymena thermophila telomerase holoenzyme protein p65 induces multiple structural changes in telomerase RNA [J] . AkiyamaB.M., LoperJ., NajarroK., RNA . 2012,第4期

机译：嗜热四膜虫端粒酶全酶蛋白p65的C端结构域诱导端粒酶RNA的多个结构变化
4. TRANSCRIPTIONAL REGULATION MECHANISMS OF TELOMERASE REVERSE TRANSCRIPTASE GENE RESPONSIBLE FOR UBIQUITOUS EXPRESSION OF TELOMERASE IN MOUSE [C] . T. Fujiki, Y. Katakura, T. Miura, Annual Meeting of the Japanese Association for Animal Cell Technology . 2003

机译：端粒酶逆转录酶基因的转录调节机制，其对小鼠端粒酶普遍存在的逆转转录酶基因
5. Roles for Terminal Uridyl Transferases in the Post-Transcriptional Regulation of Developmental miRNAs. [D] . Thornton, James Edward. 2013

机译：终端的铀酰转移酶在发育miRNA的转录后调控中的作用。
6. Telomerase can act as a template- and RNA-independent terminal transferase [O] . Neal F. Lue, Dimitry Bosoy, Tara J. Moriarty, 2005

机译：端粒酶可以充当独立于模板和RNA的末端转移酶
7. Telomerase can act as a template- and RNA-independent terminal transferase [O] . Lue, Neal F., Bosoy, Dimitry, Moriarty, Tara J., 2005

机译：端粒酶可以充当独立于模板和RNA的末端转移酶

Telomerase can act as a template- and RNA-independent terminal transferase

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