...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Isolation and functional expression of an animal geranyl diphosphate synthase and its role in bark beetle pheromone biosynthesis
【24h】

Isolation and functional expression of an animal geranyl diphosphate synthase and its role in bark beetle pheromone biosynthesis

机译：动物香叶基二磷酸合酶的分离，功能表达及其在树皮甲虫信息素生物合成中的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Geranyl diphosphate synthase (GPPS) catalyzes the condensation of dimethylallyl diphosphate and isopentenyl diphosphate to form geranyl diphosphate. Geranyl diphosphate is the precursor of monoterpenes, a large family of natural occurring C-10 compounds predominately found in plants. Similar to plants but unique to animals, some bark beetle genera (Coleoptera: Scolytidae) produce monoterpenes that function in intraspecific chemical communication as aggregation and dispersion pheromones. The release of monoterpene aggregation pheromone mediates host colonization and mating. It has been debated whether these monoterpene pheromone components are derived de novo through the mevalonate pathway or result from simple modifications of dietary precursors. The data reported here provide conclusive evidence for de novo biosynthesis of monoterpene pheromone components from bark beetles. We describe GPPS in the midgut tissue of pheromone-producing male lips pini. GPPS expression levels are regulated by juvenile hormone III, similar to other mevalonate pathway genes involved in pheromone biosynthesis. In addition, GPPS transcript is almost exclusively expressed in the anterior midgut of male L pini, the site of aggregation pheromone biosynthesis. The recombinant enzyme was functionally expressed and produced geranyl diphosphate as its major product. The three-dimensional model structure of GPPS shows that the insect enzyme has the sequence structural motifs common to E-isoprenyl diphosphate synthases.
机译：香叶基二磷酸合酶（GPPS）催化二甲基烯丙基二磷酸和异戊烯基二磷酸的缩合形成香叶基二磷酸。草甘膦二磷酸酯是单萜的前体，单萜是在植物中发现的大量天然存在的C-10化合物。与植物相似，但对动物来说却是独特的，一些树皮甲虫属（鞘翅目：鞘翅目）产生单萜，它们在种内化学通讯中起聚集和分散信息素的作用。单萜聚集信息素的释放介导宿主定植和交配。这些单萜信息素成分是通过甲羟戊酸途径从头衍生而来还是由饮食前体的简单修饰引起的，一直存在争议。此处报道的数据为从树皮甲虫中单萜信息素成分的从头生物合成提供了确凿的证据。我们在产生信息素的雄性唇松的中肠组织中描述了GPPS。 GPPS的表达水平受幼体激素III的调控，与信息素生物合成中涉及的其他甲羟戊酸途径基因相似。此外，GPPS转录本几乎只在雄性L皮尼的前中肠（聚集信息素的生物合成位点）中表达。重组酶在功能上被表达并产生了香叶基二磷酸作为其主要产物。 GPPS的三维模型结构表明，该昆虫酶具有E-异戊二烯基二磷酸合酶共有的序列结构基序。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号