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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae
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The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-beta-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae

机译：Smk1p MAP激酶在酿酒酵母的孢子壁形态发生过程中负调控Gsc2p，1，3-β-葡聚糖合酶。

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摘要

Spore formation in Saccharomyces cerevisiae involves the sequential deposition of multiple spore wall layers between the prospore membranes that surround each meiotic product. The Smk1p mitogen-activated protein (MAP) kinase plays a critical role in spore formation, but the proteins that interact with Smk1p to regulate spore morphogenesis have not been described. Using mass spectrometry, we identify Gsc2p as a Smk1p-associated protein. Gsc2p is a 1,3-beta-glucan synthase subunit involved in synthesizing an inner spore wall layer. We find that 1,3-beta-glucan synthase activity is elevated in smk1 mutants, suggesting that SMK1 negatively regulates GSC2. Although deposition of the two inner spore wall layers is normal in smk1 mutants, deposition of the outer layers is aberrant. However, eliminating GSC2 activity restores normal deposition of the third spore wall layer in smk1 mutants, indicating that negative regulation of GSC2 by SMK1 is important for spore wall deposition. Our findings suggest a model for the coordination of spore wall layer deposition in which Smk1 p facilitates the transition between early and late phases of spore wall deposition by inhibiting a spore wall-synthesizing enzyme important for early phases of spore wall deposition.
机译：酿酒酵母中的孢子形成涉及围绕每个减数分裂产物的子孢子膜之间的多个孢子壁层的顺序沉积。 Smk1p丝裂原活化蛋白（MAP）激酶在孢子形成中起关键作用，但尚未描述与Smk1p相互作用以调节孢子形态发生的蛋白。使用质谱，我们将Gsc2p识别为Smk1p相关蛋白。 Gsc2p是参与合成内部孢子壁层的1,3-β-葡聚糖合酶亚基。我们发现1,3-β-葡聚糖合酶活性在smk1突变体中升高，表明SMK1负调控GSC2。尽管在smk1突变体中两个内孢子壁层的沉积是正常的，但外层的沉积却异常。但是，消除GSC2活性可恢复smk1突变体中第三孢子壁层的正常沉积，这表明SMK1对GSC2的负调控对于孢子壁沉积很重要。我们的发现提出了一种用于协调孢子壁层沉积的模型，其中Smk1p通过抑制对孢子壁沉积早期重要的孢子壁合成酶，促进了孢子壁沉积早期阶段和晚期阶段之间的过渡。

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