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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Toward the development of peptide nanofilaments and nanoropes as smart materials
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Toward the development of peptide nanofilaments and nanoropes as smart materials

机译：致力于开发肽纳米丝和纳米绳作为智能材料

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摘要

Protein design studies using coiled coils have illustrated the potential of engineering simple peptides to self-associate into polymers and networks. Although basic aspects of self-assembly in protein systems have been demonstrated, it remains a major challenge to create materials whose large-scale structures are well determined from design of local protein-protein interactions. Here, we show the design and characterization of a helical peptide, which uses phased hydrophobic interactions to drive assembly into nanofilaments and fibrils ("nanoropes"). Using the hydrophobic effect to drive self-assembly circumvents problems of uncontrolled self-assembly seen in previous approaches that used electrostatics as a mode for self-assembly. The nanostructures designed here are characterized by biophysical methods including analytical ultracentrifugation, dynamic light scattering, and circular dichroism to measure their solution properties, and atomic force microscopy to study their behavior on surfaces. Additionally, the assembly of such structures can be predictably regulated by using various environmental factors, such as pH, salt, other molecular crowding reagents, and specifically designed "capping" peptides. This ability to regulate self-assembly is a critical feature in creating smart peptide biomaterials.
机译：使用卷曲螺旋的蛋白质设计研究表明，工程化简单肽具有自缔合成聚合物和网络的潜力。尽管已经证明了蛋白质系统中自组装的基本方面，但要创建其大规模结构由局部蛋白质-蛋白质相互作用的设计很好地确定的材料，仍然是一项重大挑战。在这里，我们展示了螺旋肽的设计和表征，该螺旋肽使用阶段性的疏水相互作用来驱动组装成纳米丝和原纤维（“纳米膜”）。使用疏水效应来驱动自组装可避免在以前使用静电作为自组装模式的方法中看到的不受控制的自组装问题。这里设计的纳米结构的特征在于生物物理方法，包括分析超离心，动态光散射和圆二色性以测量其溶液性质，以及原子力显微镜研究其在表面上的行为。另外，可以通过使用各种环境因素，例如pH，盐，其他分子拥挤试剂和专门设计的“封端”肽来可预测地调节这种结构的组装。这种调节自组装的能力是创建智能肽生物材料的关键特征。

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