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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Electrical synapses in retinal ON cone bipolar cells: Subtype-specific expression of connexins
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Electrical synapses in retinal ON cone bipolar cells: Subtype-specific expression of connexins

机译：视网膜ON锥体双极细胞中的电突触：连接蛋白的亚型特异性表达。

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Retinal bipolar cells are known to form a complex, interconnecting network through electrical synapses that are either heterologous (with amacrine cells) or homologous (with other bipolar cells). These electrical synapses can be functionally as important as chemical synapses because their distinct properties provide a different character for the network. Much less is known, however, about electrical synapses in retinal bipolar cells than about chemical synapses. Here we report the molecular basis for electrical synapses in retinal bipolar cells, particularly ON cone bipolar cells. We have found variable connexin 36 (cx36) expression in different types of ON cone bipolar cells: cx36 message was found in some, but not all, ON cone bipolar cells (4 of 14 cells). In one specific type of ON cone bipolar cell (BPGus-GFp), however, cx36 was detected in 17 of 19 cells. Moreover, we have located cx36 puncta at the axonal terminals of BPGus-GFP cells, and we have found that these BPGUS-GFP-associated cx36 puncta always colocalized with All amacrine cell processes. Molecular and immunocytochemical evidence obtained in this study also shows that connexin 45 (cx45) is not present in BPGUS-GFP cells. Taken together, our results suggest that connexins are expressed in bipolar cells in a neuronal subtype-specific manner and that cx36/cx36 gap junctions form the heterologous electrical synapses between All amacrine cells and BPGUS-GFP cells. Our findings imply that visual information can be differently processed by distinct subtypes of ON cone bipolar cells via electrical synapses.
机译：已知视网膜双极细胞通过异源（与无长突细胞）或同源（与其他双极细胞）的电突触形成复杂的互连网络。这些电突触在功能上与化学突触一样重要，因为它们的独特特性为网络提供了不同的特性。然而，关于视网膜双极细胞中的电突触的了解远少于化学突触。在这里，我们报告视网膜双极细胞，特别是圆锥双极细胞中的电突触的分子基础。我们已经在不同类型的ON锥双极细胞中发现了可变的连接蛋白36（cx36）表达：在一些但不是全部ON锥双极细胞（14个细胞中有4个）中发现了cx36信息。然而，在一种特定类型的ON锥形双极细胞（BPGus-GFp）中，在19个细胞中的17个中检测到cx36。此外，我们已经将cx36点定位在BPGus-GFP细胞的轴突末端，并且我们发现这些与BPGUS-GFP相关的cx36点始终与所有无长突细胞过程共定位。这项研究中获得的分子和免疫细胞化学证据也表明，连接蛋白45（cx45）在BPGUS-GFP细胞中不存在。综上所述，我们的结果表明连接蛋白以神经元亚型特异性的方式在双极细胞中表达，并且cx36 / cx36间隙连接形成了所有无长突细胞和BPGUS-GFP细胞之间的异源电突触。我们的发现暗示视觉信息可以通过电突触由ON锥双极细胞的不同亚型不同地处理。

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