...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats
【24h】

Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats

机译：中国马蹄蝠的严重急性呼吸系统综合症冠状病毒样病毒

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although the finding of severe acute respiratory syndrome coronavirus (SARS-CoV) in caged palm civets from live animal markets in China has provided evidence for interspecies transmission in the genesis of the SARS epidemic, subsequent studies suggested that the civet may have served only as an amplification host for SARS-CoV. In a surveillance study for CoV in noncaged animals from the wild areas of the Hong Kong Special Administration Region, we identified a CoV closely related to SARS-CoV (bat-SARS-CoV) from 23 (39%) of 59 anal swabs of wild Chinese horseshoe bats (Rhinolophus sinicus) by using RT-PCR. Sequencing and analysis of three bat-SARS-CoV genomes from samples collected at different dates showed that bat-SARS-CoV is closely related to SARS-CoV from humans and civets. Phylogenetic analysis showed that bat-SARS-CoV formed a distinct cluster with SARS-CoV as group 2b CoV, distantly related to known group 2 CoV. Most differences between the bat-SARS-CoV and SARS-CoV genomes were observed in the spike genes, ORF 3 and ORF 8, which are the regions where most variations also were observed between human and civet SARS-CoV genomes. In addition, the presence of a 29-bp insertion in ORF 8 of bat-SARS-CoV genome, not in most human SARS-CoV genomes, suggests that it has a common ancestor with civet SARS-CoV. Antibody against recombinant bat-SARSCoV nucleocapsid protein was detected in 84% of Chinese horseshoe bats by using an enzyme immunoassay. Neutralizing antibody to human SARS-CoV also was detected in bats with lower viral loads. Precautions should be exercised in the handling of these animals.
机译：尽管在中国活体动物市场的笼养棕榈科动物身上发现了严重的急性呼吸综合症冠状病毒（SARS-CoV），这为SARS流行的种间传播提供了证据，但随后的研究表明，这种灵猫可能只是作为SARS-CoV的扩增宿主。在香港特别行政区野生地区非笼养动物冠状病毒的监测研究中，我们从59个野生肛门拭子中的23个（39％）中发现了与SARS-CoV（bat-SARS-CoV）密切相关的冠状病毒通过逆转录-聚合酶链反应（RT-PCR）的中国马蹄蝙蝠（Rhinolophus sinicus）。对不同日期采集的三个蝙蝠-SARS-CoV基因组进行测序和分析表明，蝙蝠-SARS-CoV与人类和野生动植物的SARS-CoV密切相关。系统发育分析表明，bat-SARS-CoV与SARS-CoV作为2b CoV组形成了明显的簇，与已知的2 CoV组远缘相关。蝙蝠-SARS-CoV和SARS-CoV基因组之间的大多数差异在刺突基因ORF 3和ORF 8中观察到，这是在人和果子狸SARS-CoV基因组之间也观察到最多变异的区域。另外，bat-SARS-CoV基因组的ORF 8中存在29 bp的插入，而不是大多数人SARS-CoV基因组中存在，这表明它与麝猫SARS-CoV有共同的祖先。使用酶免疫法在84％的中国马蹄蝠中检测到针对重组蝙蝠-SARSCoV核衣壳蛋白的抗体。在病毒载量较低的蝙蝠中也检测到了针对人SARS-CoV的中和抗体。在处理这些动物时应采取预防措施。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号