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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Use of gene expression profiling to direct in vivo molecular imaging of lung cancer
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Use of gene expression profiling to direct in vivo molecular imaging of lung cancer

机译：基因表达谱分析在肺癌体内分子成像研究中的应用

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Using gene expression profiling, we identified cathepsin cysteine proteases as highly up-regulated genes in a mouse model of human lung adenocarcinoma. Overexpression of cathepsin proteases in these lung tumors was confirmed by immunohistochemistry and Western blotting. Therefore, an optical probe activated by cathepsin proteases was selected to detect murine lung tumors in vivo as small as 1 mm in diameter and spatially separated. We generated 3D maps of the fluorescence signal and fused them with anatomical computed tomography images to show a close correlation between fluorescence signal and tumor burden. By serially imaging the same mouse, optical imaging was used to follow tumor progression. This study demonstrates the capability for molecular imaging of a primary lung tumor by using endogenous proteases expressed by a tumor. It also highlights the feasibility of using gene expression profiling to identify molecular targets for imaging lung cancer.
机译：使用基因表达谱分析，我们在人肺腺癌的小鼠模型中将组织蛋白酶半胱氨酸蛋白酶鉴定为高度上调的基因。通过免疫组织化学和蛋白质印迹证实组织蛋白酶在这些肺部肿瘤中的过表达。因此，选择了由组织蛋白酶蛋白酶激活的光学探针，以检测直径小至1 mm并在空间上分开的鼠肺肿瘤。我们生成了荧光信号的3D图，并将它们与解剖计算机断层扫描图像融合在一起，以显示荧光信号与肿瘤负荷之间的密切相关性。通过对同一只小鼠进行连续成像，可以使用光学成像跟踪肿瘤的进展。这项研究证明了通过使用肿瘤表达的内源性蛋白酶对原发性肺部肿瘤进行分子成像的能力。它还强调了使用基因表达谱分析来鉴定肺癌影像的分子靶标的可行性。

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