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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Simple sequence repeat-based consensus linkage map of Bombyx mori.
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Simple sequence repeat-based consensus linkage map of Bombyx mori.

机译：基于简单序列重复的家蚕共有连锁图谱。

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摘要

We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. A survey of six representative silkworm strains using 2,500 (CA)n- and (CT)n-based SSR markers revealed 17-24% polymorphism, indicating a high degree of homozygosity resulting from a long history of inbreeding. Twenty-nine SSR linkage groups were established in well characterized Dazao and C108 strains based on genotyping of 189 backcross progeny derived from an F(1) male mated with a C108 female. The clustering was further focused to 28 groups by genotyping 22 backcross progeny derived from an F(1) female mated with a C108 male. This set of SSR linkage groups was further assigned to the 28 chromosomes (established linkage groups) of silkworm aided by visible mutations and cleaved amplified polymorphic sequence markers developed from previously mapped genes, cDNAsequences, and cloned random amplified polymorphic DNAs. By integrating a visible mutation p (plain, larval marking) and 29 well conserved genes of insects onto this SSR-based linkage map, a second generation consensus silkworm genetic map with a range of 7-40 markers per linkage group and a total map length of approximately 3431.9 cM was constructed and its high efficiency for genotyping and potential application for synteny studies of Lepidoptera and other insects was demonstrated.
机译：作为国际基因组计划的一部分，我们建立了518个简单的序列重复（SSR或微卫星）标记用于家蚕，这是一种在经济上和文化上都很重要的鳞翅目昆虫，它的遗传连锁图谱。使用基于2,500个（CA）n和（CT）n的SSR标记对六种代表性蚕菌株进行的调查显示，其多态性为17％至24％，表明近交历史悠久，具有高度的纯合性。二十九个SSR连锁集团建立在特征明确的Dazao和C108菌株中，基于189个回交子代的基因型，该回交子代来自F（1）雄性与C108雌性交配。通过对来自F（1）雌性与C108雄性交配的22个回交子代进行基因分型，将聚类进一步集中到28个组。这组SSR连锁基团进一步被分配到家蚕的28条染色体（已建立的连锁基团），借助可见突变和从先前定位的基因，cDNA序列和克隆的随机扩增多态性DNA开发的裂解扩增多态性序列标记。通过将可见的突变p（普通，幼虫标记）和29个昆虫的保守基因整合到此基于SSR的连锁图上，获得第二代共有家蚕遗传图，每个连锁组的范围为7-40个标记，总图长大约构建了3431.9 cM，证明了其高效的基因分型和潜在应用，对鳞翅目和其他昆虫的同性研究。

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