...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia.
【24h】

Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia.

机译：调节肾脏和视网膜上皮中单羧酸转运蛋白及其伴侣CD147的组织特异性极性的机制。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Proton-coupled monocarboxylate transporters (MCT) MCT1, MCT3, and MCT4 form heterodimeric complexes with the cell surface glycoprotein CD147 and exhibit tissue-specific polarized distributions that are essential for maintaining lactate and pH homeostasis. In the parenchymal epithelia of kidney, thyroid, and liver, MCT/CD147 heterocomplexes are localized in the basolateral membrane where they transport lactate out of or into the cell depending on metabolic conditions. A unique distribution of lactate transporters is found in the retinal pigment epithelium (RPE), which regulates lactate levels of the outer retina. In RPE, MCT1/CD147 is polarized to the apical membrane and MCT3/CD147 to the basolateral membrane. The mechanisms responsible for tissue-specific polarized distribution of MCTs are unknown. Here, we demonstrate that CD147 carries sorting information for polarized targeting of the MCT1/CD147 hetero-complexes in kidney and RPE cells. In contrast, MCT3 and MCT4 harbor dominant sorting information that cotargets CD147 to the basolateral membrane in both epithelia. RNA interference experiments show that MCT1 promotes CD147 maturation. Our results open a unique paradigm to study the molecular basis of tissue-specific polarity.
机译：质子偶联的单羧酸盐转运蛋白（MCT）MCT1，MCT3和MCT4与细胞表面糖蛋白CD147形成异源二聚体复合物，并表现出组织特异性极化分布，这对于维持乳酸和pH稳态至关重要。在肾，甲状腺和肝脏的实质上皮细胞中，MCT / CD147异源复合物位于基底外侧膜中，根据代谢条件，它们将乳酸从细胞内运出或进入细胞内。在视网膜色素上皮（RPE）中发现了乳酸盐转运蛋白的独特分布，它调节了外部视网膜的乳酸盐水平。在RPE中，MCT1 / CD147极化至顶膜，MCT3 / CD147极化至基底外侧膜。 MCT的组织特异性极化分布的机制尚不清楚。在这里，我们证明了CD147携带有针对肾脏和RPE细胞中MCT1 / CD147杂合物的极化靶向的分类信息。相比之下，MCT3和MCT4具有将CD147共同靶向两个上皮基底外侧膜的主要分类信息。 RNA干扰实验表明MCT1促进CD147的成熟。我们的结果为研究组织特异性极性的分子基础开辟了独特的范例。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号