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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship.
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Functional analysis of fruitless gene expression by transgenic manipulations of Drosophila courtship.

机译：果蝇求爱的转基因操作的无结果基因表达的功能分析。

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摘要

A gal4-containing enhancer-trap called C309 was previously shown to cause subnormal courtship of Drosophila males toward females and courtship among males when driving a conditional disrupter of synaptic transmission (shi(TS)). We extended these manipulations to analyze all features of male-specific behavior, including courtship song, which was almost eliminated by driving shi(TS) at high temperature. In the context of singing defects and homosexual courtship affected by mutations in the fru gene, a tra-regulated component of the sex-determination hierarchy, we found a C309/tra(F) combination also to induce high levels of courtship between pairs of males and "chaining" behavior in groups; however, these doubly transgenic males sang normally. Because production of male-specific FRU(M) protein is regulated by TRA, we hypothesized that a fru-derived transgene encoding the male (M) form of an Inhibitory RNA (fru(MIR)) would mimic the effects of tra(F); but C309/fru(MIR) males exhibited no courtship chaining, although they courted other males in single-pair tests. Double-labeling of neurons in which GFP was driven by C309 revealed that 10 of the 20 CNS clusters containing FRU(M) in wild-type males included coexpressing neurons. Histological analysis of the developing CNS could not rationalize the absence of tra(F) or fru(MIR) effects on courtship song, because we found C309 to be coexpressed with FRU(M) within the same 10 neuronal clusters in pupae. Thus, we hypothesize that elimination of singing behavior by the C309/shi(TS) combination involves neurons acting downstream of FRU(M) cells.
机译：先前显示，当驾驶条件性突触传递干扰物（shi（TS））时，含有gal4的增强子诱集体C309会导致果蝇雄性对雌性的恋爱不佳，以及雄性中的恋爱。我们扩展了这些操作，以分析男性特定行为的所有特征，包括求爱歌曲，这种行为在高温下驱动shi（TS）几乎被消除。在歌唱缺陷和同性恋求爱受到fru基因突变（性别决定体系中一个tra调控的组成部分）的影响的情况下，我们发现C309 / tra（F）组合还可以诱导成对男性之间的高水平求爱分组中的“链接”行为；然而，这些双重转基因雄性正常唱歌。因为TRA调节男性特异性FRU（M）蛋白的产生，所以我们假设编码编码抑制性RNA（fru（MIR）的雄性（M）形式的fru衍生的转基因将模仿tra（F）的作用;但是C309 / fru（MIR）雄性没有表现出求爱链，尽管他们在单对测试中向其他雄性求婚。 C309驱动GFP的神经元的双标记显示，野生型雄性中包含FRU（M）的20个CNS簇中有10个包含共表达神经元。发育中的中枢神经系统的组织学分析不能合理地说明对求爱歌的tra（F）或fru（MIR）的影响不存在，因为我们发现C309在F中的相同10个神经元簇中与FRU（M）共表达。因此，我们假设通过C309 / shi（TS）组合消除唱歌行为涉及神经元作用于FRU（M）细胞的下游。

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