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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test
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Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test

机译：反复强迫游泳试验后，过表达甘丙肽的小鼠海马去甲肾上腺素和血清素释放增强

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Basal and forced swimming (FS) stress-induced release of noradrenaline (NA) and serotonin (5-HT) were determined by in vivo microdialysis in the ventral hippocampus of mice overexpressing galanin under the platelet-derived growth factor B promoter (GalOE/P) or under the dopamine β-hydroxylase promoter (Ga-IOE/D) (only NA). WT mice served as controls. Intraventricular infusion of galanin significantly reduced basal extracellular NA in WT mice and in GalOE/P mice (albeit less so). Microdialysis sampling during a 10-min FS showed that NA and 5-HT release were elevated to 213% and 156%, respectively, in the GalOE/P group, whereas in the WT group the increases were only 127% and 119%, respectively. The second (repeated) 10-min FS (RFS) caused a marked enhancement of NA and 5-HT release in the GalOE/P mice to 344% and 275%, respectively. However, the RFS caused only a 192% increase of extracellular NA levels in the GalOE/D mice. Pretreatment with the putative peptidergic galanin receptor antagonist M35 almost completely blocked the elevation of NA and 5-HT levels in the GalOE/P after RFS. These results suggest that the NA and 5-HT hippocampal afferents in GalOE/P mice are hypersensitive to both conditioned and unconditioned stressful stimuli, such as FS, and that this effect is mediated by galanin receptors. The present findings support a role of galanin in the regulation of release of NA and 5-HT, two neurotransmitters involved in mood control.
机译：通过体内微透析测定血小板衍生的生长因子B启动子（GalOE / P）下过表达甘丙肽的小鼠腹侧海马中的基础和强迫游泳（FS）应激诱导的去甲肾上腺素（NA）和5-羟色胺（5-HT）释放）或在多巴胺β-羟化酶启动子（Ga-IOE / D）（仅NA）之下。 WT小鼠用作对照。脑室内注射甘丙肽可显着降低野生型小鼠和GalOE / P小鼠的基础细胞外NA（尽管影响较小）。在10分钟的FS期间进行的微透析采样显示，GalOE / P组的NA和5-HT释放分别提高至213％和156％，而WT组的分别仅为127％和119％ 。第二次（重复）10分钟FS（RFS）导致GalOE / P小鼠的NA和5-HT释放分别显着增强至344％和275％。但是，RFS只能使GalOE / D小鼠的细胞外NA水平增加192％。假定的肽能的甘丙肽受体拮抗剂M35预处理几乎完全阻断了RFS后GalOE / P中NA和5-HT水平的升高。这些结果表明，GalOE / P小鼠中的NA和5-HT海马传入细胞对条件性和非条件性应激刺激（例如FS）都高度敏感，并且这种作用是由甘丙肽受体介导的。目前的发现支持甘丙肽在调节NA和5-HT（涉及情绪控制的两种神经递质）的释放中的作用。

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