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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cell-cell signaling controls Xylella fastidiosa interactions with both insects and plants
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Cell-cell signaling controls Xylella fastidiosa interactions with both insects and plants

机译：细胞信号传导控制小木鼠与昆虫和植物的相互作用

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摘要

Xylella fastidiosa, which causes Pierce's disease of grapevine and other important plant diseases, is a xylem-limited bacterium that depends on insect vectors for transmission. Although many studies have addressed disease symptom development and transmission of the pathogen by vectors, little is known about the bacterial mechanisms driving these processes. Recently available X. fastidiosa genomic sequences and molecular tools have provided new routes for investigation. Here, we show that a diffusible signal molecule is required for biofilm formation in the vector and for vector transmission to plants. We constructed strains of X. fastidiosa mutated in the rpfF gene and determined that they are unable to produce the signal activity. In addition, rpfF mutants are more virulent than the wild type when mechanically inoculated into plants. This signal therefore directs interaction of X. fastidiosa with both its insect vector and plant host. Interestingly, rpfF mutants can still form in planta biofilms, which differ architecturally from biofilms in insects, suggesting that biofilm architecture, rather than a passive response to the environment, is actively determined by X. fastidiosa gene expression. This article reports a cell-cell signaling requirement for vector transmission. Identification of the genes regulated by rpfF should elucidate bacterial factors involved in transmission and biofilm formation in the insect.
机译：引起木耳皮尔斯氏病和其他重要植物病的木杆菌是一种木质部受限的细菌，其依赖昆虫载体进行传播。尽管许多研究已经通过载体解决了疾病症状发展和病原体传播的问题，但对于驱动这些过程的细菌机制知之甚少。最近可用的法氏假单胞菌基因组序列和分子工具提供了新的研究途径。在这里，我们表明，在载体中生物膜形成以及载体向植物的传播中需要扩散信号分子。我们构建了在rpfF基因中突变的X. fastidiosa菌株，并确定它们无法产生信号活性。另外，当机械接种到植物中时，rpfF突变体比野生型更具毒性。因此，该信号指导X.fastidiosa与其昆虫载体和植物宿主的相互作用。有趣的是，rpfF突变体仍然可以在植物的生物膜中形成，其结构与昆虫的生物膜不同，这表明生物膜的结构而不是对环境的被动响应是由X. fastidiosa基因表达主动确定的。本文报告了矢量传输的信元信令要求。鉴定由rpfF调控的基因应阐明涉及昆虫传播和生物膜形成的细菌因素。

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