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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley
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Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley

机译：鉴定将原叶绿素化物氧化还原酶A输入大麦叶绿体中所需的质体包膜蛋白

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摘要

Chloroplasts synthesize an abundance of different tetrapyrrole compounds. Among them are chlorophyll and its precursor protochlorophyllide (Pchlide), which accumulate in light- and dark-grown plants, respectively. Pchlide is converted to chlorophyllide by virtue of the NADPHiPchlide oxidoreductase (POR), which, in angiosperms, is the only known light-dependent enzyme of the chlorophyll biosynthetic pathway. In etiolated barley plants, two closely related POR proteins exist termed PORA and PORB, which are nuclear gene products. Here we identified plastid envelope proteins that interact with the cyto-solic PORA precursor (pPORA) during its posttranslational chloroplast import. We demonstrate that pPORA interacts with several previously unreported components. Among them is a Pchlide a oxygenase, which provides Pchlide b as import substrate for pPORA, and a tyrosine aminotransferase thought to be involved in the synthesis of photoprotective vitamin E. Two other constituents were found to be orthologs of the GTP-binding proteins Toc33/34 and of the outer plastid envelope protein Oep16.
机译：叶绿体合成大量不同的四吡咯化合物。其中包括叶绿素及其前体原叶绿素内酯（Pchlide），它们分别积累在浅色和深色植物中。借助NADPHiPchlide氧化还原酶（POR），可将磷脂转化为叶绿素，在被子植物中，POR是叶绿素生物合成途径中唯一已知的光依赖性酶。在黄化大麦植物中，存在两个密切相关的POR蛋白，称为PORA和PORB，它们是核基因产物。在这里，我们确定了质体包膜蛋白，它们在翻译后叶绿体​​导入过程中与胞质PORA前体（pPORA）相互作用。我们证明pPORA与几个以前未报告的组件进行交互。其中有一个Pchlide a加氧酶（提供Pchlide b作为pPORA的进口底物）和一个酪氨酸氨基转移酶（被认为与光保护性维生素E的合成有关）。另外两个成分是GTP结合蛋白Toc33 /的直系同源物。 34和外部质体包膜蛋白Oep16。

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