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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Estrogen alters hippocampal dendritic spine shape and enhances synaptic protein immunoreactivity and spatial memory in female mice
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Estrogen alters hippocampal dendritic spine shape and enhances synaptic protein immunoreactivity and spatial memory in female mice

机译：雌激素改变雌性小鼠海马树突棘的形状并增强突触蛋白的免疫反应性和空间记忆

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Estrogen (E) treatment induces axospinous synapses in rat hippocampus in vivo and in cultured hippocampal neurons in vitro. To better explore the molecular mechanisms underlying this phenomenon, we have established a mouse model for E action in the hippocampus by using Golgi impregnation to examine hippocampal dendritic spine morphology, radioimmunocytochemistry (RICC) and silver-enhanced immunocytochemistry to examine expression levels of synaptic protein markers, and hippocampal-dependent object-placement memory as a behavioral readout for the actions of E. In ovariectomized mice of several strains and F_1 hybrids, the total dendritic spine density on neurons in the CA1 region was not enhanced by E treatment, a finding that differs from that in the female rat. E treatment of ovariectomized C57BL/6J mice, however, caused an increase in the number of spines with mushroom shapes. By RICC and silver-enhanced immunocytochemistry, we found that the immunoreactivity of postsynaptic markers (PSD95 and spi-nophilin) and a presynaptic marker (syntaxin) were enhanced by E treatment throughout all fields of the dorsal hippocampus. In the object-placement tests, E treatment enhanced performance of object placement, a spatial episodic memory task. Taken together, the morphology and RICC results suggest a previously uncharac-terized role of E in synaptic structural plasticity that may be interpreted as a facilitation of the spine-maturation process and may be associated with enhancement of hippocampal-dependent memory.
机译：雌激素（E）处理可在体内和体外培养的海马神经元中诱导轴突突触。为了更好地探究此现象的分子机制，我们通过高尔基体浸渍检查了海马树突状棘突的形态，放射免疫细胞化学（RICC）和银增强免疫细胞化学来检查突触蛋白标记的表达水平，建立了海马E行为的小鼠模型。 ，以及海马依赖的对象放置记忆作为对E的行为的行为读出。在数种品系和F_1杂种的去卵巢小鼠中，E处理并未增强CA1区神经元的总树突棘密度。与雌性老鼠不同。然而，卵巢切除的C57BL / 6J小鼠的E治疗导致蘑菇形棘的数量增加。通过RICC和银增强的免疫细胞化学，我们发现E处理在整个背侧海马区域均增强了突触后标志物（PSD95和spi-nophilin）和突触前标志物（突触素）的免疫反应性。在对象放置测试中，E处理可增强对象放置的性能，这是一种空间情景记忆任务。总之，形态学和RICC结果表明E在突触结构可塑性中具有以前未曾表征的作用，这可以解释为促进脊柱成熟过程，并且可能与增强海马依赖性记忆有关。

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