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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells.
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Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells.

机译：葡萄糖刺激的小鼠胰岛β细胞中高尔基复合体不同水平下的水箱之间的直接连续性。

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摘要

Direct continuity between the membranes of cisternae in the Golgi complex in mammalian cells rarely has been observed; when seen, its documentation has been equivocal. Here we have used dual-axis electron microscope tomography to examine the architecture of the Golgi in three dimensions at approximately 6-nm resolution in rapidly frozen, freeze-substituted murine cells that make and secrete insulin in response to glucose challenge. Our data show three types of direct connections between Golgi cisternae that are normally distinct from one another. These connections all "bypass" interceding cisternae. We propose that when pancreatic beta cells are stimulated to synthesize and secrete insulin rapidly in vivo, such connections provide a continuous lumen that facilitates the rapid transit of large amounts of newly made protein for secretion. The heterotypic fusion of cisternae, even transiently, raises important questions about the molecular mechanisms that (i) facilitate the fusion/fission of cisternal membranes and control the directionality and specificity of such events, and (ii) retain Golgi processing enzymes at specific places within individual cisternae when two cisternae at different levels in the Golgi have fused, maintaining the sequential processing hierarchy that is a hallmark of Golgi organization.
机译：很少观察到哺乳动物细胞中高尔基复合体的水箱膜之间有直接的连续性。看到时，它的文档是模棱两可的。在这里，我们已经使用双轴电子显微镜断层扫描技术在快速冷冻，冷冻取代的鼠细胞中以大约6 nm的分辨率在三个维度上检查了高尔基体的结构，这些鼠细胞响应葡萄糖的刺激而产生和分泌胰岛素。我们的数据显示高尔基水箱之间的三种直接连接类型通常彼此不同。这些连接全部“绕过”插入水箱。我们提出，当胰腺β细胞被刺激合成并在体内快速分泌胰岛素时，这种连接提供了连续的内腔，从而促进了大量新近分泌的蛋白质的快速转运。池的异型融合，即使是短暂的融合，也提出了有关以下分子机制的重要问题：（i）促进脑池膜的融合/裂变并控制此类事件的方向性和特异性，（ii）在高尔基体内的特定位置保留高尔基加工酶高尔基体中两个不同级别的池体融合在一起时，将保持单个池体，从而保持顺序处理层次结构，这是高尔基体组织的标志。

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