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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination.
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Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination.

机译：硫磺菌种的三个复制起点：染色体复制的同步启动和异步终止。

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摘要

Chromosome replication origins were mapped in vivo in the two hyperthermophilic archaea, Sulfolobus acidocaldarius and Sulfolobus solfataricus, by using microarray-based marker frequency analysis. Bidirectional replication was found to be initiated in near synchrony from three separate sites in both organisms. Two of the three replication origins in each species were located in the vicinity of a cdc6/orc1 replication initiation gene, whereas no known replication-associated gene could be identified near the third origin in either organism. In contrast to initiation, replication termination occurred asynchronously, such that certain replication forks continued to progress for >40 min after the others had terminated. In each species, all replication forks advanced at similar DNA polymerization rates; this was found to be an order of magnitude below that displayed by Escherichia coli and thus closer to eukaryotic elongation rates. In S. acidocaldarius, a region containing short regularly spaced repeats wasfound to hybridize aberrantly, as compared to the rest of the chromosome, raising the possibility of a centromere-like function.
机译：通过使用基于微阵列的标记频率分析，在两个嗜热古细菌Sulfolobus acidocaldarius和Sulfolobus solfataricus中体内绘制了染色体复制起点。发现双向复制是从两种生物中的三个独立位点几乎同步开始的。每个物种的三个复制起点中的两个位于cdc6 / orc1复制起始基因附近，而在任何一个生物体的第三个起点附近都找不到与复制相关的基因。与启动相反，复制终止是异步发生的，因此某些复制叉在其他终止之前继续进行了40分钟以上。在每个物种中，所有复制叉都以相似的DNA聚合速率前进。发现这比大肠杆菌显示的数量级低一个数量级，因此更接近真核生物的延伸率。在嗜酸链球菌中，与其他染色体相比，发现包含短而规则间隔的重复序列的区域异常杂交，从而增加了着丝粒样功能的可能性。

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