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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The mechanism of transmembrane S-nitrosothiol transport.
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The mechanism of transmembrane S-nitrosothiol transport.

机译：跨膜S-亚硝基硫醇转运的机制。

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S-nitrosothiols have been suggested to play an important role in nitric oxide (NO)-mediated biological events. However, the mechanisms by which an S-nitrosothiol (or the S-nitroso functional group) is transferred across cell membrane are still poorly understood. We have demonstrated previously that the degradation of S-nitrosoglutathione (GSNO) by cells absolutely required the presence of cystine in the extracellular medium and proposed a mechanism that involved the reduction of cystine to cysteine, followed by the reaction of cysteine with GSNO to form S-nitrosocysteine (CysNO), mixed disulfides, and nitrosyl anion. In the present study we have assessed the effect of cystine on the transfer of the S-nitroso functional group from the extracellular to the intracellular space. Using RAW 264.7 cells, we found that the presence of l-cystine enhanced GSNO-dependent S-nitrosothiol uptake, increasing the intracellular S-nitrosothiol level from approximately 60 pmol/mg of protein to approximately 3 nmol/mg of protein. The uptake seems to depend on the reduction of l-cystine to l-cysteine, which involves the x(c)(-) amino acid transport system, the transnitrosation between GSNO and l-cysteine to form l-CysNO, and uptake of l-CysNO via amino acid transport system L. Compared with GSNO, (Z)-1-[N-(3-ammoniopropyl)-N-[4-(3-aminopropylammonio)butyl]-amino]diazen- 1-ium-1,2-diolate, an NO donor, is much less effective at intracellular S-nitrosothiol formation in the presence of l-cystine or l-cysteine, suggesting that the biochemical changes that occur after exposure of cells to S-nitrosothiol, with respect to thiol chemistry, are distinctly different from those observed with NO.
机译：已建议S-亚硝基硫醇在一氧化氮（NO）介导的生物事件中起重要作用。然而，关于S-亚硝基硫醇（或S-亚硝基官能团）跨细胞膜转移的机制仍知之甚少。先前我们已经证明，细胞对S-亚硝基谷胱甘肽（GSNO）的降解绝对需要在细胞外培养基中存在半胱氨酸，并提出了一种机制，其中涉及将半胱氨酸还原为半胱氨酸，然后使半胱氨酸与GSNO反应形成S -亚硝基半胱氨酸（CysNO），混合的二硫化物和亚硝酰基阴离子。在本研究中，我们评估了胱氨酸对S-亚硝基官能团从细胞外空间向细胞内空间转移的影响。使用RAW 264.7细胞，我们发现l-胱氨酸的存在增强了GSNO依赖性S-亚硝基硫醇的摄取，使细胞内S-亚硝基硫醇的含量从大约60 pmol / mg蛋白质增加到大约3 nmol / mg蛋白质。摄取似乎取决于将l-半胱氨酸还原为l-半胱氨酸，这涉及x（c）（-）氨基酸转运系统，GSNO和l-半胱氨酸之间的亚硝基化以形成l-CysNO，以及l的摄取。 -CysNO通过氨基酸转运系统L。与GSNO相比，（Z）-1- [N-（3-氨丙基）-N- [4-（3-氨丙基氨）丁基]-氨基]重氮-1-ium-1 1,2-羟乙酸盐（一种NO供体）在存在L-胱氨酸或L-半胱氨酸的情况下对细胞内S-亚硝基硫醇的形成作用要差得多，这表明相对于细胞暴露于S-亚硝基硫醇后发生的生化变化硫醇化学与用NO观察到的明显不同。

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