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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Heterodimerization of type Ⅱ phytochromes in Arabicdopsis
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Heterodimerization of type Ⅱ phytochromes in Arabicdopsis

机译：拟南芥中Ⅱ型植物色素的异二聚化作用

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Coimmunoprecipitation of members of the phytochrome red/far-red photoreceptor family from plant extracts has been used to analyze their heteromeric binding interactions. Phytochrome (phy)B or phyD apoproteins with six myc epitopes fused to their N termini are biologically active when expressed in Arabidopsis. Immunoprecipitation of either of these tagged proteins from seedling extracts coprecipitates additional type Ⅱ phytochromes: six myc (myc_6)-phyB coprecipitates phyC-phyE; and myc_6-phyD coprecipitates phyB and phyE. No interaction of the epitope-tagged proteins with type Ⅰ phyA was detected. Gel filtration chromatography shows that all five of the Arabidopsis phytochromes are present in seedlings as dimers, and that the heteromeric type Ⅱ phytochrome complexes migrate at molecular masses characteristic of heterodimers. Similar levels of heterodimer formation are observed in extracts of dark-grown seedlings, where the phytochromes are cytosolic, and light-grown seedlings, where they are predominantly nuclear. These findings indicate that Arabidopsis, which until now has been thought to contain five ho-modimeric forms of phytochrome, in fact contains multiple species of both homodimeric and heterodimeric phytochromes. The conservation of the phytochrome family throughout angiosperms suggests that heterodimeric red/far-red receptors may be present in many flowering plants.
机译：来自植物提取物的植物色素红/远红感光体家族成员的共免疫沉淀已用于分析其异聚体结合相互作用。当在拟南芥中表达时，带有六个myc表位融合到其N末端的植物色素（phy）B或phyD载脂蛋白具有生物活性。从幼苗提取物中对这两种标记蛋白进行免疫沉淀会共同沉淀出其他的Ⅱ型植物色素：六个myc（myc_6）-phyB会共同沉淀phyC-phyE。和myc_6-phyD共沉淀phyB和phyE。未检测到带有表位标记的蛋白与Ⅰ型phyA的相互作用。凝胶过滤色谱分析表明，所有五个拟南芥植物色素都以二聚体形式存在于幼苗中，并且Ⅱ型植物色素的杂聚体复合物以异二聚体的分子质量进行迁移。在深色植物幼苗的提取物中观察到相似的异二聚体形成水平，其中植物色素是胞质的，而浅色幼苗则主要是核的。这些发现表明，直到现在仍被认为含有五种ho-modimeric形式的植物色素的拟南芥，实际上含有多种同二聚体和异二聚体植物色素。整个被子植物中植物色素家族的保守性表明，许多开花植物中可能存在异二聚体红/远红受体。

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