...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation.
【24h】

Hematopoietic cells and osteoblasts are derived from a common marrow progenitor after bone marrow transplantation.

机译：造血细胞和成骨细胞来自骨髓移植后的普通骨髓祖细胞。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bone and bone marrow are closely aligned physiologic compartments, suggesting that these tissues may represent a single functional unit with a common bone marrow progenitor that gives rise to both osteoblasts and hematopoietic cells. Although reports of multilineage engraftment by a single marrow-derived stem cell support this idea, more recent evidence has challenged claims of stem cell transdifferentiation and therefore the existence of a multipotent hematopoietic/osteogenic progenitor cell. Using a repopulation assay in mice, we show here that gene-marked, transplantable marrow cells from the plastic-nonadherent population can generate both functional osteoblasts/osteocytes and hematopoietic cells. Fluorescent in situ hybridization for the X and Y chromosomes and karyotype analysis of cultured osteoblasts confirmed the donor origin of these cells and excluded their generation by a fusion process. Molecular analysis demonstrated a common retroviral integration site in clonogenic hematopoietic cells and osteoprogenitors from each of seven animals studied, establishing a shared clonal origin for these ostensibly independent cell types. Our findings indicate that the bone marrow contains a primitive cell able to generate both the hematopoietic and osteocytic lineages. Its isolation and characterization may suggest novel treatments for genetic bone diseases and bone injuries.
机译：骨骼和骨髓是紧密排列的生理区室，表明这些组织可能代表具有共同的骨髓祖细胞的单个功能单元，而骨髓祖细胞同时产生成骨细胞和造血细胞。尽管单个骨髓干细胞进行多系移植的报道支持这一观点，但最近的证据挑战了干细胞转分化的主张，因此挑战了多能造血/成骨祖细胞的存在。使用小鼠中的再种群试验，我们在这里显示了来自非粘附塑料群体的基因标记的可移植骨髓细胞可以同时生成功能性成骨细胞/成骨细胞和造血细胞。 X和Y染色体的荧光原位杂交以及培养的成骨细胞的核型分析证实了这些细胞的供体来源，并通过融合过程排除了它们的产生。分子分析表明，在所研究的七只动物的每一个中，克隆形成的造血细胞和骨祖细胞中都有一个常见的逆转录病毒整合位点，为这些表面上独立的细胞类型建立了共同的克隆起源。我们的发现表明，骨髓中含有一种能够生成造血和骨细胞谱系的原始细胞。它的分离和表征可能为遗传性骨病和骨损伤的治疗提出新的建议。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号