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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Type II platelet-activating factor-acetylhydrolase is essential for epithelial morphogenesis in Caenorhabditis elegans.
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Type II platelet-activating factor-acetylhydrolase is essential for epithelial morphogenesis in Caenorhabditis elegans.

机译：II型血小板活化因子-乙酰水解酶对于秀丽隐杆线虫的上皮形态发生是必不可少的。

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摘要

Type II platelet-activating factor-acetylhydrolase [PAF-AH (II)] is an N-myristoylated enzyme that contains a lipase/esterase catalytic motif and selectively hydrolyzes the sn-2 acetyl ester of PAF and other short-chain acyl groups attached to phosphoglycerides. However, the physiological role of this enzyme remains to be elucidated. PAF-AH (II) is conserved in a variety of species ranging from a simple multicellular organism, Caenorhabditis elegans, to mammals. C. elegans possesses two homologous PAF-AH (II) genes, named paf-1 and paf-2. In this study, we generated these two loss-of-function mutants to elucidate the in vivo PAF-AH (II) function. Surprisingly, mutants of paf-2, a major isoform of C. elegans PAF-AH (II)s, exhibits gross defects in epithelial sheet formation, resulting in unsuccessful subsequent morphogenesis with complete penetrance. Moreover, paf-2 RNA interference worms show a variable abnormal morphology, including ectopic protrusions and a lumpy shape at the late embryonic and earlylarval stages due to epithelial organization defects. Consistent with these phenotypes, PAF-AH (II) is predominantly expressed in epithelial cells of C. elegans. This study demonstrates that PAF-AH (II) is essential for epithelial morphogenesis.
机译：II型血小板活化因子乙酰水解酶[PAF-AH（II）]是一种N-肉豆蔻酰化酶，它含有脂肪酶/酯酶催化基序，并选择性水解PAF的sn-2乙酰基酯和连接到其上的其他短链酰基磷酸甘油酯。但是，该酶的生理作用仍有待阐明。 PAF-AH（II）在从简单的多细胞生物秀丽隐杆线虫到哺乳动物的多种物种中均得到保存。秀丽隐杆线虫具有两个同源的PAF-AH（II）基因，分别称为paf-1和paf-2。在这项研究中，我们生成了这两个功能丧失的突变体，以阐明体内PAF-AH（II）的功能。令人惊讶地，秀丽隐杆线虫PAF-AH（II）的主要同种型paf-2的突变体在上皮层形成中显示出明显的缺陷，导致随后的形态发生不成功，并具有完全的渗透性。此外，由于上皮组织缺陷，paf-2 RNA干扰蠕虫在胚胎后期和幼虫早期阶段表现出可变的异常形态，包括异位突起和块状。与这些表型一致，PAF-AH（II）主要在秀丽隐杆线虫的上皮细胞中表达。这项研究表明，PAF-AH（II）对于上皮形态发生是必不可少的。

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