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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis.
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A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis.

机译：转录共激活因子AtGIF1参与调节拟南芥的叶片生长和形态。

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摘要

Previously, we described the AtGRF [Arabidopsis thaliana growth-regulating factor (GRF)] gene family, which encodes putative transcription factors that play a regulatory role in growth and development of leaves and cotyledons. We demonstrate here that the C-terminal region of GRF proteins has transactivation activity. In search of partner proteins for GRF1, we identified another gene family, GRF-interacting factor (GIF), which comprises three members. Sequence and molecular analysis showed that GIF1 is a functional homolog of the human SYT transcription coactivator. We found that the N-terminal region of GIF1 protein was involved in the interaction with GRF1. To understand the biological function of GIF1, we isolated a loss-of-function mutant of GIF1 and prepared transgenic plants subject to GIF1-specific RNA interference. Like grf mutants, the gif1 mutant and transgenic plants developed narrower leaves and petals than did wild-type plants, and combinations of gif1 and grf mutations showed a cooperative effect. The narrow leaf phenotype of gif1, as well as that of the grf triple mutant, was caused by a reduction in cell numbers along the leaf-width axis. We propose that GRF1 and GIF1 act as transcription activator and coactivator, respectively, and that they are part of a complex involved in regulating the growth and shape of leaves and petals.
机译：以前，我们描述了AtGRF [拟南芥生长调节因子（GRF）]基因家族，该家族编码假定的转录因子，在叶片和子叶的生长和发育中起调节作用。我们在这里证明，GRF蛋白的C末端区域具有反式激活活性。为了寻找GRF1的伴侣蛋白，我们鉴定了另一个基因家族，即GRF相互作用因子（GIF），它包含三个成员。序列和分子分析表明，GIF1是人SYT转录共激活因子的功能同源物。我们发现，GIF1蛋白的N末端区域参与了与GRF1的相互作用。为了了解GIF1的生物学功能，我们分离了GIF1的功能丧失突变体，并制备了受到GIF1特异性RNA干扰的转基因植物。像grf突变体一样，gif1突变体和转基因植物的叶子和花瓣比野生型植物窄，并且gif1和grf突变的组合显示出协同作用。 gif1的窄叶表型以及grf三重突变体的表型是由沿叶宽轴的细胞数减少引起的。我们建议GRF1和GIF1分别充当转录激活因子和共激活因子，并且它们是参与调节叶和花瓣的生长和形状的复合体的一部分。

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