...
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles
【24h】

Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles

机译:基于与未修饰金纳米粒子的静电相互作用比色检测DNA序列

获取原文
获取原文并翻译 | 示例
           

摘要

We find that single- and double-stranded oligonucleotides have different propensities to adsorb on gold nanoparticles in colloidal solution. We use this observation to design a hybridization assay based on color changes associated with gold aggregation. Because the underlying adsorption mechanism is electrostatic, no covalent functionalization of the gold, the probe, or the target DNA is required. Hybridization conditions can be optimized because it is completely separated from the detection step. The assay is complete within 5 min, and < 100 femtomoles of target produces color changes observable without instrumentation. Single-base-pair mismatches are easily detected.
机译:我们发现单链和双链寡核苷酸具有不同的吸附在胶体溶液中的金纳米颗粒上的倾向。我们使用这种观察来设计基于与金聚集相关的颜色变化的杂交测定。由于潜在的吸附机制是静电的,因此不需要金,探针或目标DNA的共价官能化。由于杂交条件与检测步骤完全分离,因此可以优化杂交条件。该测定法在5分钟内完成,并且在不使用仪器的情况下,小于100飞摩尔的目标产生可观察到的颜色变化。单碱基对不匹配很容易检测到。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号